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  • Microsatellite  (10)
Collection
Keywords
Years
  • 1
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    Genetic relationships of Iranian coastline ship sturgeon (Acipenser nudiventris) samples and Ural population based on microsatellite DNA (2018)
    Details
    Publication Date: 2021-05-19
    Description: This article has no abstract.
    Description: Published
    Keywords: Acipenser nudiventris ; DNA ; Genetic ; Populations ; Microsatellite
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.229-240
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  • 2
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    Isolation and characterization of microsatellite loci in the Persian sturgeon (Acipenser persicus, Borodine, 1897) and cross-species amplification in four commercial sturgeons from the Caspian Sea (2018)
    Details
    Publication Date: 2021-05-19
    Description: In order to have a sustainable management on Persian sturgeon as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea.
    Description: Published
    Keywords: Russian sturgeon ; A. gueldenstaedtii ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Population genetic ; Genetics ; Population ; Structure ; Species
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.548-558
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  • 3
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    Application of microsatellite markers to determine populations of the Persian sturgeon (Acipenser persicus) (2018)
    Details
    Publication Date: 2021-05-19
    Description: The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 – 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci ( Ls 68 , Spl 168 , Spl 173 and Afu 68 ) were analyzed for the molecular characterization of this species which resulted in polymorphic patterns. DNA bands were analysed using Biocapt and GenAlex software package. A total of 109 alleles were observed of which the maximum number of alleles (17) were found in Spl168 locus which belonged to sturgeons from Sefidrud river,s watershed and the minimum number of alleles (10) in Ls68 locus belonging to the sturgeons from Gorganrud river,s watershed. Results of microsatellite analysis revealed that the differences between samples of two regions were not statistically significant (p〉0.05), neither for the average number of alleles per locus nor for observed heterozygosities. The calculated Fst and Rst between two regions was 0.07 and 0.17 showing that the genetic difference was significant (p〈 0.01). Samples from Sefidrud river,s watershed in Spl173, Afu68 and Spl168 loci and samples of other regions in Afu68 and Spl168 loci were at Hardy-Weinberg equation. The genetic distance was calculated as 0.4 which represents a significant genetic difference between samples of two studied areas. I n conclusion, this study suggests that the Persian sturgeons in two regions of the southern part of the Caspian Sea are genetically differentiated, therefore fisheries management of these unique stocks for restocking and conservation of gene pools is highly recommended.
    Description: Published
    Keywords: Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Genetic structure ; Population genetic ; Fisheries
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.596-606
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  • 4
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    Application of microsatellite markers to study the genetic structure of stellate sturgeon populations (Acipenser stellatus Pallas, 1771) in the south Caspian Sea (2018)
    Details
    Publication Date: 2021-05-19
    Description: Population genetic structure of stellate sturgeon (Acipenser stellatus) in 197 adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 was monomorph. Totally, 184 alleles were identified and on average 13.1 alleles per locus were found (ranged 8 to 18 alleles). All sampled regions contained unique alleles. Average observed and expected heterozygosity was 0.667 and 0.854, respectively and significant genetic differences between 4 regions were observed (p£0.01). Deviations from Hardy-Weinberg equilibrium were found in most cases. Population differentiation test was modest and significant (p£0.01). Based on FST estimate (p£0.01), more than one population of stellate sturgeon is identified in the south Caspian Sea. Therefore, fishery management for restocking and conservation of gene pool is highly recommended.
    Description: Published
    Keywords: Acipenser stellatus ; Stellate sturgeon ; Population genetic ; Microsatellite ; Genetic ; Structure ; Populations
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.73-84
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  • 5
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    Genetic variability and differentiation of common Kilka fish (Clupeonella cultriventris Nordmann, 1840) in the southern coasts of Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/21944 | 18721 | 2018-01-18 08:55:31 | 21944 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: A total of 120 samples of adult common Kilka fish (Clupeonella cultriventris) were collected during spring and summer from the southern coasts of Caspian Sea (Bandar Anzali and Babolsar). Fifteen sets of microsatellite primers were developed from Clupeidae being tested on genomic DNA of common Kilka. Allele frequency, observed and expected heterozygosity, FST, RST, FIS index were determined. Five primer sets as polymorphic loci were used to analyze the genetic variation in adults of the common Kilka population. Results revealed that average alleles per locus was 13.1 (range 5 to 22 alleles per locus in regions, Ne=9.5). All sampled regions contained private alleles. Average observed and expected heterozygosity was 0.348 and 0.877, respectively. Deviations from Hardy Weinberg equilibrium were observed in most cases. FST, RST and gene flow estimates in AMOVA and the genetic distance between populations indicated that the genetic difference among the studied populations was pronounced. The data generated in this study provide primary information on the genetic variation and differentiation in populations of Caspian common Kilka.
    Keywords: Biology ; Fisheries ; Population ; genetic ; Microsatellite ; Clupeidae ; Common kilka ; Clupeonella cultriventris ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 139-148
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  • 6
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    Application of microsatellite markers to determine populations of the Persian sturgeon (Acipenser persicus) in the south of Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/22465 | 18721 | 2018-04-10 21:21:52 | 22465 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-06
    Description: The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 - 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci (Ls68, Spl168, Spl173 and Afu68) were analyzed for the molecular characterization of this species which resulted in polymorphic patterns. DNA bands were analysed using Biocapt and GenAlex software package. A total of 109 alleles were observed of which the maximum number of alleles (17) were found in Spl168 locus which belonged to sturgeons from Sefidrud river's watershed and the minimum number of alleles (10) in Ls68 locus belonging to the sturgeons from Gorganrud river's watershed. Results of microsatellite analysis revealed that the differences between samples of two regions were not statistically significant (p〉0.05), neither for the average number of alleles per locus nor for observed heterozygosities. The calculated Fst and Rst between two regions was 0.07 and 0.17 showing that the genetic difference was significant (p〈 0.01). Samples from Sefidrud river's watershed in Spl173, Afu68 and Spl168 loci and samples of other regions in Afu68 and Spl168 loci were at Hardy-Weinberg equation. The genetic distance was calculated as 0.4 which represents a significant genetic difference between samples of two studied areas. In conclusion, this study suggests that the Persian sturgeons in two regions of the southern part of the Caspian Sea are genetically differentiated, therefore fisheries management of these unique stocks for restocking and conservation of gene pools is highly recommended.
    Description: Article includes abstract in Farsi on last page.
    Keywords: Biology ; Fisheries ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Genetic structure ; Population genetic ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 596-606
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  • 7
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    Unknown
    Isolation and characterization of microsatellite loci in the Persian sturgeon (Acipenser persicus, Borodine, 1897) and cross-species amplification in four commercial sturgeons from the Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/22534 | 18721 | 2018-04-23 11:01:55 | 22534 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-07
    Description: In order to have a sustainable management on Persian sturgeon as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A. nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea.
    Keywords: Biology ; Fisheries ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Population genetic ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 548-558
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  • 8
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    Unknown
    Application of microsatellite markers to study the genetic structure of stellate sturgeon populations (Acipenser stellatus Pallas, 1771) in the south Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/22336 | 18721 | 2018-03-22 17:51:18 | 22336 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-03
    Description: Population genetic structure of stellate sturgeon (Acipenser stellatus) in 197 adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 was monomorph. Totally, 184 alleles were identified and on average 13.1 alleles per locus were found (ranged 8 to 18 alleles). All sampled regions contained unique alleles. Average observed and expected heterozygosity was 0.667 and 0.854, respectively and significant genetic differences between 4 regions were observed (p≤0.01). Deviations from Hardy-Weinberg equilibrium were found in most cases. Population differentiation test was modest and significant (p≤0.01). Based on FST estimate (p≤0.01), more than one population of stellate sturgeon is identified in the south Caspian Sea. Therefore, fishery management for restocking and conservation of gene pool is highly recommended.
    Keywords: Biology ; Fisheries ; Stellate sturgeon ; Population genetics ; Microsatellite ; South Caspian Sea ; Iran ; Acipenser stellatus
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 73-84
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  • 9
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    Unknown
    Genetic variability and differentiation of common Kilka fish (Clupeonella cultriventris Nordmann, 1840) in the southern coasts of Caspian Sea (2017)
    Details
    Publication Date: 2021-05-19
    Description: A total of 120 samples of adult common Kilka fish (Clupeonella cultriventris) were collected during spring and summer from the southern coasts of Caspian Sea (Bandar Anzali and Babolsar). Fifteen sets of microsatellite primers were developed from Clupeidae being tested on genomic DNA of common Kilka. Allele frequency, observed and expected heterozygosity, FST, RST, FIS index were determined. Five primer sets as polymorphic loci were used to analyze the genetic variation in adults of the common Kilka population. Results revealed that average alleles per locus was 13.1 (range 5 to 22 alleles per locus in regions, Ne=9.5). All sampled regions contained private alleles. Average observed and expected heterozygosity was 0.348 and 0.877, respectively. Deviations from HardyWeinberg equilibrium were observed in most cases. FST, RST and gene flow estimates in AMOVA and the genetic distance between populations indicated that the genetic difference among the studied populations was pronounced. The data generated in this study provide primary information on the genetic variation and differentiation in populations of Caspian common Kilka.
    Description: Published
    Keywords: Clupeidae ; Kilka ; Clupeonella cultriventris ; Population ; Genetic ; Microsatellite ; Fish ; DNA
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.139-148
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  • 10
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    Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran (2017)
    Details
    Publication Date: 2021-05-19
    Description: This study was conducted in order to investigate genetic diversity and population structure of pike-perch in the Northern part of Iran. For this purpose, 207 adult pike-perches from four regions of the Caspian Sea watershed (Talesh Coasts, Anzali Wetland, Chaboksar Coasts and Aras Dam) were collected. DNA was extracted and by using 15 pairs of microsatellite primers, Polymerase Chain Reaction (PCR) was conducted. DNA bands were analyzed using Biocapt and GenAlex 6 software package. Out of 15 microsatellite primers, 11 loci were produced, of those, 6 loci were polymorphic and 5 were monomorphic. Analysis revealed that the average number of alleles per locus and observed heterozygosities were not statistically significant (P〉0.05) for all four populations. Data indicated an appreciable genetic differentiation, in spite of a low genetic variation, and agreed with the low level of genetic polymorphism already observed for this species in Iran. Deviation from Hardy-Weinberg equilibrium was obvious in most cases, mostly due to the deficiency of heterozygosities. The highest genetic distance was between Anzali Wetland and Aras Dam populations. This investigation represents the first approach to the knowledge of the genetic variability of Iranian populations using microsatellite markers, and reported results could be of interest for future management and conservation programs of this species in Iran
    Description: Published
    Keywords: Sander lucioperca L. ; Pike-perch ; Sander lucioperca ; Genetic analysis ; Microsatellite ; Genetic ; Populations ; Satellite ; DNA ; Marketing
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.99-108
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