ISSN:
1573-5044
Keywords:
Agrobacterium tumefaciens
;
genetic transformation
;
GUS
;
Mentha spicata
;
Mentha arvensis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The stable integration of GUS and NPTII genes in Mentha arvensis and M. spicata has been achieved by Agrobacterium tumefaciens-mediated gene transfer. Transformation assays were performed by cocultivating plant leaf disks with either GV2260/GI or EHA105/MOG Agrobacterium strains. Transgenic plants were selected on medium containing 150 mg l−1 kanamycin. Transgene presence and structure was studied by the use of PCR analysis and Southern blot hybridization. Transgene expression was evaluated by RT-PCR and transgene product activity by a histoenzymatic GUS assay.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006280521212
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