ISSN:
1615-6102
Keywords:
Higher plant
;
Immunofluorescence
;
Meiosis
;
Microtubule organizing centers
;
Preprophase band
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A monoclonal antibody to higher plant tubulin was used to trace microtubule (MT) structures by immunofluorescence throughout mitosis and meiosis in two angiosperms,Lycopersicon esculentum andOrnithogalum virens. Root tip cells showed stage specific MT patterns typical of higher plant cells. These included parallel cortical interphase arrays oriented perpendicular to the long axis of the cell, preprophase band MTs in late interphase through prophase, barrelshaped spindles, and finally phragmoplasts. Pollen mother cell divisions exhibited randomly oriented cortical MT arrays in prophase I, pointed spindles during karyokinesis, and elongate phragmoplasts. A preprophase band was not observed in either meiotic division. MT initiation sites were seen as broad zones associated with the nuclear envelope.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01281021
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