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Investigation of chloroplast DNA heteroplasmy in Medicago sativa L. using cultured tissue (1993)

Fitter, J. T. ; Rose, R. J.

Springer

Theoretical and applied genetics 86 (1993), S. 65-70

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ISSN: 1432-2242

Keywords: Chloroplast DNA ; Heteroplasmy ; Medicago sativa ; Tissue culture ; Preferential replication

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Medicago sativa L. cv Regen S is heteroplasmic for chloroplast DNA (cpDNA). Previous analyses of regenerated plants have shown a predominance of one of the cpDNAs which we have designated type A (the other we have designated type B). Studies of the replication of the two cpDNAs in tissue culture were carried out using leaflet expiants with defined cpDNA types and a distinguishing probe. The explants obtained showed a bias toward type A cpDNA during tissue culture. The data suggest that chloroplasts with different DNAs in a common nuclear background can multiply at different rates.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00223809

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