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  • Mating-type switching  (1)
  • Spheroplast and lithium chloride transformation  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Hybridization and cytoduction among yeast cells of the same mating type (1987)
    Springer
    Current genetics 12 (1987), S. 511-517 
    Details
    ISSN: 1432-0983
    Keywords: Mating-type switching ; Cytoduction ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Use of a selective system for cytoduction in Saccharomyces cerevisiae allowed us to monitor hybrid formation and to clone the haploid nuclei of cells which have participated in illegitimate matings: a × a, α × α. Our approach has made it possible to select nuclei with mating-type switches and mutations within the MAT locus. It was shown that matings in α × α crosses often proceed through nonheritable genetic changes located within chromosome III. We suggest that these non-heritable genetic changes are due to premutational lesions, expressed phenotypically as transient α-matingtype. After a mating event these lesions are either repaired or converted to true mutations within the MAT locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419560
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid genetic test for discrimination between haploid and polyploid transformants in Saccharomyces (1984)
    Springer
    Current genetics 8 (1984), S. 341-344 
    Details
    ISSN: 1432-0983
    Keywords: Spheroplast and lithium chloride transformation ; Mitotic homozygotization ; Polyploid transformants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spheroplast fusion induced by polyethylene glycol often occurs during transformation of yeast spheroplasts. It leads to the appearance of polyploid transformants. We have developed a method of discriminating between haploid and polyploid transformants, based on qualitative comparison of the frequency of mitotic homozygotization for a recessive marker, lys2, in diploid and polyploid hybrids of transformants crossed with a tester haploid bearing lys2. We have used this method to compare the frequency of appearance of polyploids during transformations of spheroplasts and of intact cells using lithium chloride in the strain DC5. Polyploids constitute from 15% to 74% of transformants after transformation of spheroplasts. In lithium chloride transformation polyploids arose in only one of four experiments and constituted 9% of transformants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419822
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    Paper (German National Licenses)
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