ISSN:
0935-6304
Keywords:
Capillary GC
;
Mass spectrometry
;
Fish oil supplementation
;
Furanpropionic acid
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
3-Carboxy-4-methyl-5-propyl-2-furanpropionic acid (furanpropionic acid), a metabolite commonly found in human blood and urine, is one of the major acidic components which accumulates in the blood of patients with renal failure. Polyunsaturated fatty acids such as eicosapentaenoic acid (C20:5 ω-3) and long chain furan fatty acids, both present in commonly available fish oil capsules, are regarded as possible precursors of furanpropionic acid. Because of its strong binding to albumin and its various interactions with other endogenous and exogenous components, it is of great importance to determine the concentration of furanpropionic acid in blood and urine regularly.Solid phase extraction has been used to concentrate furanpropionic acid from both blood and urine samples. After esterification with ethereal diazomethane, gas chromatographic separation was performed on a HP-1 column (dimethylpolysiloxane). Quantitation with an internal standard was performed by mass selective detection in SIM-mode, qualifier ions being used for identification and accuracy.Fish oil supplementation of the diet of 24 patients for four weeks led to 3-fold and 5 to 6-fold increases in the levels of furanpropionic acid in serum and urine, respectively.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240151209
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