ISSN:
1420-9071
Keywords:
Prothoracicotropic hormone
;
Manduca sexta
;
anti-PTTH monoclonal antibody
;
sedimentation coefficient
;
frictional ratio
;
molecular weight
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The prothoracicotropic hormones (PTTHs) are cerebral peptides that control insect postembryonic development by stimulating the prothoracic glands to synthesize ecdysteroids. InManduca sexta, the tobacco hornworm, two classes of PTTH are distinguished by their Mr, small (ca. 7 kDa) and big PTTH (ca. 25–30 kDa). Little is known about the physical nature of the PTTHs and this study takes a first step towards defining characteristics of theManduca big PTTH. The neurohormone has a Stokes radius of 2.59 nm and a sedimentation coefficient of 2.76 S. Based on these data, an Mr of 29,443.7 and anf/f 0 of 1.27 were calculated. Combined, the physical data revealManduca big PTTH is an asymmetrical acidic homodimeric peptide with intra- and intermolecular disulfide bonds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01984956
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