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  • 1
    Electronic Resource
    Electronic Resource
    DNA markers linked to Malus floribunda 821 scab resistance (1994)
    Springer
    Plant molecular biology 26 (1994), S. 597-602 
    Details
    ISSN: 1573-5028
    Keywords: apple ; DNA marker ; RAPD ; Venturia inaequalis ; Malus floribunda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Breeding resistant apple plants is an alternative way to control fungal pathogens reducing the environmental impact due to the use of pesticides. The breeding of apple cultivars resistant to Venturia inaequalis could be much improved by marker-assisted selection. A molecular marker closely linked to the resistance locus called Vf could replace selection based on infection studies. To find such molecular markers, DNA of progenies from crossings of a resistant and a susceptible apple tree was subject to bulked segregant analysis. Two markers were found with a genetic distance of 10.6% and 19.7% recombination frequency to the Vf locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00013746
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular selection in apple for resistance to scab caused by Venturia inaequalis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 199-204 
    Details
    ISSN: 1432-2242
    Keywords: Malus ; Molecular markers ; Apple scab ; Plant breeding ; Marker assisted selection ; Venturia inaequalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Large-scale marker-assisted selection requires highly reproducible, consistent and simple markers. The use of genetic markers is important in woody plant breeding in general, and in apple in particular, because of the high level of heterozygosity present in Malus species. We present here the transformation of two RAPD markers, which we found previously to be linked to the major scab resistance gene Vf, into more reliable and reproducible markers that can be applied directly to apple breeding. We give an example of how the use of such markers can speed up selection for the introduction of scab resistance genes into the same plant, reducing labour and avoiding time-consuming test crosses. We discuss the nature and relationship of the scab resistance gene Vf to the one present in Nova Easygro, thought to be Vr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225746
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  • 3
    Electronic Resource
    Electronic Resource
    Simple sequence repeats for the genetic analysis of apple (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1069-1076 
    Details
    ISSN: 1432-2242
    Keywords: Key words SSR ; Microsatellite ; Plant breeding ; Molecular markers ; Germplasm ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The development of highly informative markers, such as simple sequence repeats, for tagging genes controlling agronomic characters is essential for apple breeding. Furthermore the use of these markers is fundamental both for variety identification and for the characterisation and management of genetic resources. We have developed 16 reliable simple sequence repeat (SSR) markers that amplify all alleles from a panel of 19 Malus x domestica (Borkh.) cultivars or breeding selections and from Malus floribunda 821. Those markers show a high level of genetic polymorphism, with on average 8.2 alleles per locus and an average heterozygosity of 0.78. Due to this high level of polymorphism, it was possible using two selected SSRs to distinguish all cultivars except Starking and Red Delicious. Ten of the markers we developed have been mapped on a RAPD linkage map, proving their Mendelian segregation as well as their random distribution in the apple genome. Finally, we discuss the importance of using co-dominant markers in outbreeding species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050841
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular selection in apple for resistance to scab caused by Venturia inaequalis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 199-204 
    Details
    ISSN: 1432-2242
    Keywords: Key words Malus ; Molecular markers ; Apple scab ; Plant breeding ; Marker assisted selection ; Venturia inaequalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Large-scale marker-assisted selection requires highly reproducible, consistent and simple markers. The use of genetic markers is important in woody plant breeding in general, and in apple in particular, because of the high level of heterozygosity present in Malus species. We present here the transformation of two RAPD markers, which we found previously to be linked to the major scab resistance gene Vf, into more reliable and reproducible markers that can be applied directly to apple breeding. We give an example of how the use of such markers can speed up selection for the introduction of scab resistance genes into the same plant, reducing labour and avoiding time-consuming test crosses. We discuss the nature and relationship of the scab resistance gene Vf to the one present in Nova Easygro, thought to be Vr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050266
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  • 5
    Electronic Resource
    Electronic Resource
    Towards the map-based cloning of Vf: fine and physical mapping of the Vf Region (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 1012-1017 
    Details
    ISSN: 1432-2242
    Keywords: Key words Malus ×domestica ; Venturia inaequalis ; Malus floribunda ; Disease resistance ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A map-based cloning scheme is being used to isolate the Vf resistance gene of apple against the fungus Venturia inaequalis. Vf is a major dominant gene that is inherited in a Mendelian manner and influenced by minor genes that modify its activity. The two recently published markers M18 and AL07, bracketing Vf, were tested on 1179 progeny plants of three crosses to fine-map Vf. M18 and AL07 were positioned at 0.2 cM and 1.1 cM from Vf respectively, for a total distance between the two markers of 1.3 cM. Physical mapping by pulsed-field gel electrophoresis, using M18 and AL07 as probes, demonstrated that both markers hybridize to a common 870 kb NotI restriction fragment. We therefore found a relationship between physical and genetic distance of 670 kb/cM in the Vf region. This led us to the conclusion that a chromosome walk using the recently published apple BAC library starting from M18 and AL07 is feasible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051409
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