ISSN:
1618-2545
Keywords:
ectomycorrhizal infection
;
Hartig net
;
inoculation method
;
Pinus densiflora
;
Tricholoma matsutake
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The root systems of 11-wk-oldPinus densiflora seedlings were inoculated with a hyphal suspension ofTricholoma matsutake and aseptically incubated for 4 wk in a forest soil without supplying exogenous carbohydrates. One week following inoculation, fungal hyphae had colonized the root surface and bound soil particles together establishing a root-substrate continuum. Fungal hyphae were visible within the main root cortex following clearing bleaching and staining. In the ensuing days, fungal colonization was observed within elongating lateral roots in which Hartig net formation was confirmed 4 wk after inoculation. This is the first report of rapid ectomycorrhizal infection ofP. densiflora seedings byT. matsutake.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02463952
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