Publication Date:
2021-05-19
Description:
Today, Bisphenol A is an industrial important chemical that is abundantly used as a primary
raw material for the production of plastics and resin. BPA reaches the aquatic environment
mainly through urban and industrial sewage effluents; thereby posing a potential threat to the
organisms living in these ecosystems. In this study, effects of BPA onerythrocytic nuclear
abnormalities (ENA) stimulus (MN Test) and liver DNA integrity (DNA Unwinding Assay), in
male yellowfinseabream(Acanthopagruslatus) were investigated. For this reason, fish received
intraperitoneal injections during a period of 2 weeks with 10, 50, 100 and 150 µg g-1 week-1
of
BPA dissolved in coconut oil.Solventcontrols received the coconut oil whereas controls were
not injected. The fish were sampled on day 0, 7 and 14. In order to evaluate cytotoxicity of
BPA presented in blood, the erythrocytic nuclear abnormalities (ENA) frequency in
yellowfinseabrem was determined.Our results demonstrated a significant increase in fish
micronuclei frequency after the treatment with bisphenol A in comparison to the control groups
and in dose dependent manner. In addition, the rate of liver DNA integrity was tested using the
DNA alkaline unwinding assay. Results showed a decrease in the rate of liver DNAintegrityin
treated fishes after 7 and 14 days of BPA exposure in comparison to the control group.In
conclusion, results of the current project indicated that BPA has highgenotoxic
and/orcytogenotoxic potential.It could be concluded that Micronucleus test and DNA strand
breaks can be used as sensitive cellular and molecular indicators of exposure to genotoxic BPA.
Description:
Published
Keywords:
Bis-Phenol A
;
Genotoxicology
;
MN Test
;
DNA Unwinding
;
Acanthopagruslatus
;
Levels
;
Molecular
;
Cellular
;
Yellowfin Seabream
Repository Name:
AquaDocs
Type:
Journal Contribution
,
Refereed
Format:
pp.20-30
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