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  • MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT  (1)
  • Microprojectile bombardment  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A combined use of microprojectile bombardment and DNA imbibition enhances transformation frequency of canola (Brassica napus L.) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 187-192 
    Details
    ISSN: 1432-2242
    Keywords: Canola ; Desiccation/DNA imbibition ; Microprojectile bombardment ; Microspore-derived embryos ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Efforts to increase the frequency of recovered homozygous transgenic B. napus plants from direct DNA transformation treatments led to the development of a method of combined microprojectile bombardment and desiccation/DNA imbibition. The combined method was compared to individual treatments in two experiments utilizing microspore-derived embryo hyocotyls as targets for the β-glucuronidase (GUS) and NPT II genes. Both the transient gene expression of β-GUS and the stable transformation by NPT II demonstrated that the combined use of microprojectile bombardment and desiccation/DNA imbibition yielded more transgenic plants (at least three-times more) than either individual transformation protocol. In a histochemical analysis for β-GUS activity, an average of 37% of the hypocotyls receiving the combined treatment displayed a positive response, whereas only 8% of the hypocotyls showed a positive response following microprojectile bombardment alone. The hypocotyls obtained by the joint treatment also showed more multisite expression of the β-GUS gene per hypocotyl than those treated only with microprojectile bombardment. Southern analysis of NPT II gene integration into subsequently-derived secondary embryos indicated that the transformation efficiency of the combined treatment was 2% in comparison to 0.6% for that of the singular microprojectile bombardment. The number of inserts integrating per transformation event appears to be independent of the transformation methods. Neither of the marker genes was expressed in hypocotyls treated only with desiccation/DNA imbibition. Utilization of hypocotyl regeneration from microspore-derived embryos via a secondary embryogenesis system provided a reliable method for producing transgenic plants. The combined use of microprojectile bombardment and desiccation/DNA imbibition proved to be an efficient approach to obtain homozygous transgenic canola plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225896
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    Paper (German National Licenses)
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  • 2
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    Redundant manipulators for momentum compensation in a micro-gravity environment (1988)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: This paper is concerned with the implementation and assessment of joint motion management strategies for kinematically redundant robotic manipulators operating in the micro-gravity environment of Space Station. These robots must be capable of conducting experiments and manufacturing processes without disturbing the micro-gravity environment through base reactions/motions. The redundant degrees of freedom of the manipulator permit the inverse kinematic problem to be solved simultaneously with the minimization of a cost function. The cost function in this case is the weighted sum of the squares of the base forces and moments and is minimized over discrete time segments. The Generalized Inverse Method and Rayleigh Ritz technique are used to solve the combined optimization/inverse kinematics problem. Numerical examples include various robotic configurations and degrees of manipulator redundancy.
    Keywords: MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT
    Type: AIAA PAPER 88-4121 , AIAA Guidance, Navigation and Control Conference; Aug 15, 1988 - Aug 17, 1988; Minneapolis, MN; United States
    Format: text
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    NASA TECHNICAL REPORTS
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