ISSN:
1432-2048
Keywords:
Key words: Actin
;
Actin bundling protein
;
Lilium (pollen tube)
;
Pollen tube
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. A plant 135-kDa actin-bundling protein (P-135-ABP) isolated from pollen tubes of Lilium longiflorum (Thunb.) binds stoichiometrically to F-actin filaments and bundles them in vitro (E. Yokota et al., 1998, Plant Physiol. 116: 1421–1429). To further understand the mechanism of actin-filament bundle formation by P-135-ABP, the polarity of each F-actin filament in bundles was examined using myosin subfragment 1 (S-1). Dissociation of F-actin filaments from bundles organized by P-135-ABP was induced by S-1. However, F-actin filaments that remained in a bundle and decorated by S-1 showed uniform polarity. These results indicate that P-135-ABP arranges F-actin filaments into bundles with uniform polarity and consequently plays a key role in the orientation of cytoplasmic streaming in pollen tubes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004250050631
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