Publication Date:
1987-03-27
Description:
Neoplastic tumors of the ocular lens of vertebrates do not naturally occur. Transgenic mice carrying a hybrid gene comprising the murine alpha A-crystallin promoter (-366 to +46) fused to the coding sequence of the SV40 T antigens developed lens tumors, which obliterated the eye cavity and even invaded neighboring tissue, thus establishing that the lens is not refractive to oncogenesis. Large-T antigen was detected early in lens development; it elicited morphological changes and specifically interfered with differentiation of lens fiber cells. Both alpha- and beta-crystallins persisted in many of the lens tumor cells, while gamma-crystallin was selectively reduced. Accessibility, characteristic morphology, and defined protein markers make this transparent epithelial eye tissue a potentially useful system for testing tumorigenicity of oncogenes and for studying malignant transformation from its inception until death of the animal.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mahon, K A -- Chepelinsky, A B -- Khillan, J S -- Overbeek, P A -- Piatigorsky, J -- Westphal, H -- New York, N.Y. -- Science. 1987 Mar 27;235(4796):1622-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3029873" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, Polyomavirus Transforming
;
Antigens, Viral, Tumor/analysis
;
Cell Transformation, Neoplastic
;
Cell Transformation, Viral
;
Chimera
;
Crystallins/analysis
;
Eye Neoplasms/*pathology
;
Female
;
Lens Diseases/*pathology
;
Lens, Crystalline/growth & development
;
Mice/*genetics
;
Oncogene Proteins, Viral/analysis
;
Phenotype
;
Pregnancy
;
Simian virus 40
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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