ISSN:
1573-0603
Keywords:
Conjugation
;
oriT
;
Mobilization
;
Lactic acid bacteria
;
Lactococci
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009759525576
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