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  • 1
    ISSN: 1573-0603
    Keywords: Conjugation ; oriT ; Mobilization ; Lactic acid bacteria ; Lactococci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-07-12
    Description: The Proximity-1 Space Link Interleaved Time Synchronization (PITS) protocol provides time distribution and synchronization services for space systems. A software prototype implementation of the PITS algorithm has been developed that also provides the test harness to evaluate the key functionalities of PITS with simulated data source and sink. PITS integrates time synchronization functionality into the link layer of the CCSDS Proximity-1 Space Link Protocol. The software prototype implements the network packet format, data structures, and transmit- and receive-timestamp function for a time server and a client. The software also simulates the transmit and receive-time stamp exchanges via UDP (User Datagram Protocol) socket between a time server and a time client, and produces relative time offsets and delay estimates.
    Keywords: Man/System Technology and Life Support
    Type: NPO-47404 , NASA Tech Briefs, December 2012; 9
    Format: application/pdf
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  • 3
    Publication Date: 2019-07-12
    Description: This work discusses research on the problems of synchronizing and distributing time information between spacecraft based on the Network Time Protocol (NTP), where NTP is a standard time synchronization protocol widely used in the terrestrial network. The Proximity-1 Space Link Interleaved Time Synchronization (PITS) Protocol was designed and developed for synchronizing spacecraft that are in proximity where proximity is less than 100,000 km distant. A particular application is synchronization between a Mars orbiter and rover. Lunar scenarios as well as outer-planet deep space mother-ship-probe missions may also apply. Spacecraft with more accurate time information functions as a time-server, and the other spacecraft functions as a time-client. PITS can be easily integrated and adaptable to the CCSDS Proximity-1 Space Link Protocol with minor modifications. In particular, PITS can take advantage of the timestamping strategy that underlying link layer functionality provides for accurate time offset calculation. The PITS algorithm achieves time synchronization with eight consecutive space network time packet exchanges between two spacecraft. PITS can detect and avoid possible errors from receiving duplicate and out-of-order packets by comparing with the current state variables and timestamps. Further, PITS is able to detect error events and autonomously recover from unexpected events that can possibly occur during the time synchronization and distribution process. This capability achieves an additional level of protocol protection on top of CRC or Error Correction Codes. PITS is a lightweight and efficient protocol, eliminating the needs for explicit frame sequence number and long buffer storage. The PITS protocol is capable of providing time synchronization and distribution services for a more general domain where multiple entities need to achieve time synchronization using a single point-to-point link.
    Keywords: Man/System Technology and Life Support
    Type: NPO-47403 , NASA Tech Briefs, December 2011; 26
    Format: application/pdf
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