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A series of laboratory exercises utilizing luxR gene of Vibrio fischeri and gfp gene of Aequoria victoria to teach the broad applications of polymerase chain reaction (2000)

Slock, J A ; Farrugia, P M ; Horn, T A ; [et al.]

Springer

Journal of industrial microbiology and biotechnology 24 (2000), S. 345-352

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ISSN: 1476-5535

Keywords: Keywords: polymerase chain reaction (PCR); lux bioluminescence; green fluorescence protein (GFP); directional cloning; fusion protein

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Three experiments are described; directional cloning of the luxR gene from the bioluminescent marine bacterium, Vibrio fischeri, directional cloning of the gfpgene from the marine jelly fish, Aequoria victoria, and the construction of a LuxR-GFP fusion protein. Experiments are presented using lux and gfp in an undergraduate biology curriculum. Journal of Industrial Microbiology & Biotechnology (2000) 24, 345–352.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/sj.jim.2900803

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