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  • Key wordsArxula adeninivorans  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Integrative transformation of the dimorphic yeast Arxula adeninivorans LS3 based on hygromycin B resistance (1998)
    Springer
    Current genetics 33 (1998), S. 157-163 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsArxula adeninivorans ; Transformation ; Homologous integration ; Hygromycin B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A transformation system has been developed for the dimorphic yeast Arxula adeninivorans based on a stable integration of the donor DNA into ribosomal DNA. For this purpose a cassette was constructed which contains the E. coli hph gene, conferring hygromycin B resistance, fused to the 5′ expression signals of the A. adeninivorans TEF1 gene, encoding the translation elongation factor EF-1α, and the transcription termination region of the Saccharomyces cerevisiae PHO5 gene. This cassette was fused into the 25S rDNA of A. adeninivorans. Linearization of this vector was required for high transformation frequencies. The vector was integrated in multiple copies into the 25S rDNA by homologous recombination. Copy number was not altered even after the growth of transformants for 15 generations under non-selective growth conditions. Microscopical analyses revealed that integration of the transformed plasmid did not influence the dimorphism, which is triggered at 42°C for both transformed and non-transformed cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050322
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and expression of the ARFC3 gene, a component of the replication factor C from the salt-tolerant, dimorphic yeast Arxula adeninivorans LS3 (1999)
    Springer
    Current genetics 35 (1999), S. 8-13 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsArxula adeninivorans ; ARFC3 ; Dimorphism ; Osmotolerance ; Replication factor C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The yeast replication factor C (RF-C) is a multi-polypeptide complex with five sub-units involved in chromosomal DNA replication. This factor, encoded by five genes, is well characterized for the yeast Saccharomyces cerevisiae. However, RF-C components from other yeast species have not been analysed yet. Here we report the cloning and characterization of ARFC3 from the dimorphic and osmo-tolerant yeast Arxula adeninivorans. This gene encodes one subunit of the RF-C complex. It is localized on chromosome 1 of the four Arxula chromosomes and comprises a coding region of 1014 bp, which corresponds to 338 amino acids. Two introns are contained within this gene. The ARFC3 transcript level is influenced by both salt and temperature. The latter also influences the morphological state (budding cells, mycelium). High salt concentration and high temperature result in a rapid decrease of the ARFC3 mRNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050426
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    Paper (German National Licenses)
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