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  • 1
    Electronic Resource
    Electronic Resource
    Transgenic peppermint (Mentha×piperita L.) plants obtained by cocultivation with Agrobacterium tumefaciens (1998)
    Springer
    Plant cell reports 17 (1998), S. 165-171 
    Details
    ISSN: 1432-203X
    Keywords: Key words Peppermint ; Transformation ; Agrobacterium ; GUS ; Transgenic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first transgenic peppermint (Mentha×piperita L. cultivar Black Mitcham) plants have been obtained by Agrobacterium-mediated transformation by cocultivation with morphogenically responsive leaf explants. Basal leaf explants with petioles, from leaves closest to the apex of in-vitro-culture-maintained shoots (5 cm), exhibited optimal shoot organogenetic responsiveness on medium supplemented with thidiazuron (8.4 µm). Shoot formation occurred at sites of excision on the leaf blade and petiole either directly from cells of the explant or via a primary callus. Analyses of transient GUS activity data indicated that DNA delivery by microprojectile bombardment was more effective than Agrobacterium infection. However, no transgenic plants were obtained from over 22,000 leaf explants after particle bombardment. Cocultivation of leaf explants with Agrobacterium strain EHA 105 and kanamycin selection produced transgenic plants. Greater transient and stable -glucuronidase (GUS) activities were detected in explants or propagules transformed with the construct where gusA was driven by the pBISN1 promoter rather than a CaMV 35S promoter. Eight plants were subsequently regenerated and verified as transgenic based on detection of the nptII transgene by PCR and Southern blot analyses. The Southern analyses indicated that the plants were derived from eight unique transformation events. All transgenic plants appeared morphologically normal. Analyses of GUS activities in leaves sampled from different portions of these transgenic plants, 10 months after transfer to the greenhouse, indicated that six out of the eight original regenerants were uniformly transformed, i.e., did not exhibit chimeric sectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050372
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  • 2
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    Photodisintegration of ^{7} Li with progeny nuclei in excited states (2015)
    American Physical Society (APS)
    Details
    Publication Date: 2015-10-14
    Description: Author(s): W. A. Wurtz, R. E. Pywell, B. E. Norum, S. Kucuker, B. D. Sawatzky, H. R. Weller, S. Stave, and M. W. Ahmed We study the reaction channels Li 7 + γ → n + Li 6 ( 2.19 ) ,   Li 7 + γ → n + Li 6 ( 3.56 ) , and Li 7 + γ → d + He 5 ( 1.27 ) → n + d + He 4 by detecting neutrons produced by photodisintegration events. We find absolute cross sections and angular dependence for Li 7 + γ → n + Li 6 ( 2.19 ) at photon energies 13 and 15 MeV and for Li 7 + γ → n + Li 6 ( 3.56 ) at … [Phys. Rev. C 92, 044603] Published Mon Oct 12, 2015
    Keywords: Nuclear Reactions
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
    Published by American Physical Society (APS)
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  • 3
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    Photodisintegration of Li6 (2014)
    American Physical Society (APS)
    Details
    Publication Date: 2014-07-29
    Description: Author(s): W. A. Wurtz, R. E. Pywell, B. E. Norum, S. Kucuker, B. D. Sawatzky, H. R. Weller, S. Stave, and M. W. Ahmed The cross sections for the photodisintegration of Li6 have been measured for all the available reaction channels that have neutrons in the final state, except the Li6(γ,p0)He5(g.s.) channel. The cross sections were measured at the photon energies, 8, 9, 10, 11, 12, 13, 15, and 15.6 MeV using linearl... [Phys. Rev. C 90, 014613] Published Fri Jul 25, 2014
    Keywords: Nuclear Reactions
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
    Published by American Physical Society (APS)
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