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  • 1
    Electronic Resource
    Electronic Resource
    Electron paramagnetic resonance and ultraviolet-visible spectroscopic evidence for copper porphyrin in actinomycete melanins (1998)
    Springer
    Biology and fertility of soils 26 (1998), S. 341-345 
    Details
    ISSN: 1432-0789
    Keywords: Key words Copper porphyrin ; Actinomycete melanins ; Electron paramagnetic resonance spectroscopy ; Brazilian soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The coordination chemistry of iron (III) (Fe3+) and copper (II) (Cu2+) in melanins synthesized by seven actinomycetes isolated from Brazilian latosol soils under savanna (cerrado) vegetation was investigated using electron paramagnetic resonance (EPR) and ultraviolet-visible (UV-Vis) spectroscopy. The EPR spectra indicated the presence of Cu2+ ions bound to oxygenated and nitrogenous functional groups, and rhombic coordinated Fe3+ ions. In some of the actinomycete melanins the EPR hyperfine splitting of Cu+2 ion complexes was well resolved, and indicated four magnetically equivalent nitrogen atoms in a plane. This result suggested the presence of Cu+2 porphyrin complexes, which was confirmed by Soret bands in the 400-nm region of the UV-Vis spectra. The concentration of Mn in actinomycete melanins, determined by inductively coupled plasma atomic emission spectroscopy, was lower than those of Cu and Fe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050386
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  • 2
    Electronic Resource
    Electronic Resource
    A comparative study of four different staining methods for estimation of live yeast form cells ofParacoccidioides brasiliensis (1993)
    Springer
    Mycopathologia 124 (1993), S. 157-161 
    Details
    ISSN: 1573-0832
    Keywords: Cell viability ; Paracoccidioides brasiliensis ; Staining method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A comparative study of four different staining methods for estimation of live yeast form cells ofParacoccidioides brasiliensis was carried out. The staining methods used were fluorescent staining, vital dye exclusion tests with erythrosin B and by Janus green and lactophenol cotton blue staining. Colony forming units (cfu) of the yeast form of eightP. brasiliensis isolates on brain heart infusion agar (BHIA) supplemented with 4% horse serum plus 5%P. brasiliensis cell extract (BHIA + HS + EXT) were examined for reliability of staining in determining the number of live fungal units in eight different isolates. Cfu on BHIA + HS + EXT plates showed an excellent plating efficiency over 96% in all isolates tested. The percentage of the live cells indicated by fluorescent staining (FL) or vital dye exclusion test with erythrosin B (EB) or Janus green (JG-1) was lower than that of cfu. By contrast, the percentage due to modified dye exclusion test with Janus green (JG-2) and that due to lactophenol cotton blue staining (LPCB) showed a close correration to that of cfu. Our results indicate that the modified dye exclusion test with Janus green and lactophenol cotton blue staining are useful for estimating cell viability of yeast form cells ofP. brasiliensis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103733
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