ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

β1-Integrins in the cartilage matrix (1999)

Shakibaei, M. ; Merker, Hans-Joachim

Springer

Cell & tissue research 296 (1999), S. 565-573

add to mindlist on the mindlist

Details

ISSN: 1432-0878

Keywords: Key words Immunohistology ; Immunoprecipitation ; Integrins ; Organoid culture ; Cartilage matrix ; Mouse

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Integrins are cell-surface receptors that mediate cell attachment to extracellular matrix components. The pericellular matrix in cartilage not only is a mechanical framework, but is also important for chondrocyte differentiation and stabilization of the phenotype. The interaction between chondrocytes and pericellular matrix is mediated, in part, by integrin receptors. We have previously demonstrated the presence of β1-integrins in the cartilage matrix of organoid culture of limb buds from 12-day-old mouse embryos by immunohistological methods. In order to corroborate these findings, we have further investigated the distribution of integrins in the cartilage matrix by immunoelectron microscopy and by immunoprecipitation methods. Cartilage tissue of limb buds of 17-day-old mouse embryos was treated with collagenase and the cell-free and cellular protein-free supernatant was removed and used for immunoprecipitation experiments. Immunoprecipitation with antibodies against β1-, α1-, α3-, and α5β1-integrins and collagen type II, followed by immunoblotting with the same antibodies, demonstrated the presence of these integrins and collagen type II in the supernatant. The integrins found in the cartilage matrix could have been either secreted or shed by the cells. The question as to whether they have a function in the cartilage matrix, such as interlinking, in the matrix organization or in the stabilization of matrix components remains to be elucidated.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004410051318

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Morphology of the basement membrane (1994)

Merker, Hans-Joachim

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 28 (1994), S. 95-124

add to mindlist on the mindlist

Details

ISSN: 1059-910X

Keywords: Basement membrane ; Lamina densa ; Lamina lucida ; Lamina fibroreticularis ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: The aim of this contribution is to summarize our knowledge of the morphology of the basement membrane (BM). The first step in this direction is the attempt to define this term. The BM is composed of the Lamina lucida, densa, and fibroreticularis. Subsequently, the historical development of this term is discussed. Our main interest is, of course, focused on the description of the BM-structure up to the macromolecular level and the special forms of this structure. This is supplemented by discussing its chemical composition and establishing a relationship between morphology and biochemistry. The obtained findings yielded some indications as to the molecular composition of the BM which may serve for the construction of “models.” The composition of the Lamina lucida (L.l.) and the Lamina or Pars fibroreticularis (L.f.) must be discussed separately, since, if present, they show a different and strongly varying structure (L.f.). An important aspect is the function of this extracellular layer which comprises mechanical tasks up to inductive effects. Finally, the concepts of the formation of the BM, especially of the Lamina densa (L.d.), are summarized. It obviously consists of a sequence of individual steps which starts with expression and secretion of the L.d.-components and is followed by an induction of integrin expression. © 1994 Wiley-Liss, Inc.

Additional Material: 14 Ill.