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  • 1
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of nerve growth factor (NGF) in the submandibular gland of adult mice by light and electron microscopy (1976)
    Springer
    Cell & tissue research 169 (1976), S. 289-299 
    Details
    ISSN: 1432-0878
    Keywords: Nerve growth factor ; Submandibular gland mice ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nerve growth factor (NGF) was localized in the submandibular gland of adult male mice by a direct immunocytochemical method using highly purified antibodies against NGF coupled to horseradish peroxidase. In light microscopic sections the reaction product was entirely confined to the cells of the secretory tubules. The acinar part of the gland was free of reaction product. This finding was confirmed by electron microscopy. Within the cells NGF was localized exclusively in the apical secretory granules. No reaction was observed in the rough endoplasmic reticulum, the Golgi region or in the granules of the basal part of the cells. This observation favours the assumption that NGF is derived from a precursor molecule and that the precursor is transformed into immunologically active NGF within the secretory granules during their transport from the basal to the apical part of the tubular cells. Stimulation of the submandibular gland with carbachol (2 mg/kg) led to a massive release of the content of the secretory granules, including NGF, into the salivary duct.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219602
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  • 2
    Electronic Resource
    Electronic Resource
    Asymmetric diblock copolymers–phase behaviour and kinetics of structure formation (1997)
    Springer
    Colloid & polymer science 275 (1997), S. 341-351 
    Details
    ISSN: 1435-1536
    Keywords: Key words Block copolymers ; disorder-to-order transition ; micelles ; BCC order ; kinetics of structure formation ; small angle X-ray scattering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  The structure of a series of three molecular weights of diblock copolymers polystyrene/b-isoprene with 11% volume fraction of polystyrene and low polydispersity has been investigated using small angle X-ray scattering (SAXS). In between the disordered and the BCC ordered state a micellar state with liquid-like order was found. The transition between these states was investigated in a temperature-driven experiment. Whereas the micelles appear gradually with lowering temperature the formation of the BCC ordered state occurs discontinuously at a well-defined temperature. Detailed analysis of the scattering profiles provides access to the micellar size and distance in the liquid-like as well as in the BCC state. The kinetics of the ordering transition was studied using time-resolved SAXS after temperature jumps from the liquid-like to the BCC state. The growth of the micelles and their ordering on the periodic lattice were found to occur on clearly separated time scales.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003960050091
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