ISSN:
1432-041X
Keywords:
Key words Biotin-UTP
;
Alkaline phosphatase substrates
;
Fast Red
;
Fast Blue B
;
Magenta-phos/INT
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004270050055
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