Publication Date:
2022-05-25
Description:
Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Cell Press for personal use, not for redistribution. The definitive version was published in Molecular Cell 67 (2017): 282-293, doi:10.1016/j.molcel.2017.06.018.
Description:
The eukaryotic genome is organized within cells as chromatin. For proper information
output, higher-order chromatin structures can be regulated dynamically. How such
structures form and behave in various cellular processes remains unclear. Here, by
combining super-resolution imaging (photoactivated localization microscopy, PALM)
and single nucleosome tracking, we developed a nuclear imaging system to visualize the
higher-order structures along with their dynamics in live mammalian cells. We
demonstrated that nucleosomes form compact domains with a peak diameter of ~160
nm and move coherently in live cells. The heterochromatin-rich regions showed more
domains and less movement. With cell differentiation, the domains became more
apparent, with reduced dynamics. Furthermore, various perturbation experiments
indicated that they are organized by a combination of factors, including cohesin and
nucleosome–nucleosome interactions. Notably, we observed the domains during mitosis,
suggesting that they act as building blocks of chromosomes and may serve as
information units throughout the cell cycle.
Description:
This work
was supported by MEXT and JSPS grants (23115005 and 16H04746, respectively) and
a JST CREST grant (JPMJCR15G2).
Description:
2018-07-13
Keywords:
Chromatin
;
Chromosome
;
Chromatin domain
;
PALM
;
Chromatin dynamics
;
Single nucleosome tracking
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
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