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  • Nuclear envelope  (2)
  • Key words: Nephrocytes, insect – Microfilaments – Microtubules – Junctional structures –Ceratitis capitata (Insecta)  (1)
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  • 1
    ISSN: 1432-0878
    Keywords: Mitosis ; Nuclear envelope ; Interleukin ; Development, ontogenetic ; Drosophila melanogaster (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Whole-mounts of Drosophila embryos were stained with the monoclonal antibody Vmp 18, raised against the peptide 199–208 of murine interleukin 1/α. Immunofluorescence observations showed that the antibody cross-reacted with an antigenic determinant that changed in localization during Drosophila development. In syncytial Drosophila embryos, the antibody recognized an epitope localized on the nuclear envelope throughout mitotic division. As cellularization occurred, the fluorescence was mainly concentrated in the apical region of the blastoderm cells. Western blot analysis of whole Drosophila embryo extracts showed that the antibody recognized a 60-kDa protein in syncytial embryos and during germ band elongation. This suggests that the 60-kDa antigen undergoes dynamic redistribution during embryogenesis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Key words: Mitosis ; Nuclear envelope ; Interleukin ; Development ; ontogenetic ; Drosophila melanogaster (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Whole-mounts of Drosophila embryos were stained with the monoclonal antibody Vmp 18, raised against the peptide 199–208 of murine interleukin 1/α. Immunofluorescence observations showed that the antibody cross-reacted with an antigenic determinant that changed in localization during Drosophila development. In syncytial Drosophila embryos, the antibody recognized an epitope localized on the nuclear envelope throughout mitotic division. As cellularization occurred, the fluorescence was mainly concentrated in the apical region of the blastoderm cells. Western blot analysis of whole Drosophila embryo extracts showed that the antibody recognized a 60-kDa protein in syncytial embryos and during germ band elongation. This suggests that the 60-kDa antigen undergoes dynamic redistribution during embryogenesis.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 275 (1994), S. 529-536 
    ISSN: 1432-0878
    Keywords: Key words: Nephrocytes, insect – Microfilaments – Microtubules – Junctional structures –Ceratitis capitata (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Nephrocytes are cells involved in the regulation of the composition of the hemolymph and are characterized by peripheral finger-like projections delimiting a labyrinthine channel system. We have combined structural and immunological methods to examine the spatial distribution of microtubules and microfilaments in disseminated nephrocytes of Ceratitis capitata larva; the nephrocytes are scattered among the fat-body cells, close to the salivary glands. Actin filaments are localized in two discrete peripheral domains and microtubules are mostly concentrated in the cortical region. We discuss the possibility that these cytoskeletal elements, localized in the finger-like processes of the plasma membrane, are involved in maintaining the spatial architecture of the cell periphery and in modifying the junctional complexes that represent the entrance to the labyrinthine channel system.
    Type of Medium: Electronic Resource
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