ISSN:
1432-2048
Keywords:
Key words: F1F0-ATPase inhibitor protein – Green fluorescent protein – Mitochondrion –Oryza
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. Two cDNA clones encoding F1F0-ATPase inhibitor proteins, which are loosely associated with the F1 part of the mitochondrial F1F0-ATPase, were characterized from rice (Oryza sativa L. cv. Nipponbare). A Northern hybridization showed that the two genes (designated as IF 1 -1 and IF 1 -2) are transcribed in all the organs examined. However, the steady-state mRNA levels varied among organs. A comparison of the deduced amino acid sequences of the two IF 1 genes and the amino acid sequence of the mature IF1 protein from potato revealed that IF1-1 and IF1-2 have N-terminal extensions with features that are characteristic of a mitochondrial targeting signal. To determine the subcellular localization of the gene products, the IF1-1 or IF1-2 proteins were fused in frame to the green fluorescent protein (GFP) or the fused GFP-β-glucuronidase, and expressed transiently in onion or dayflower epidermal cells. Localized fluorescence was detected in mitochondria, confirming that the two IF1 proteins are targeted to mitochondria.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008125
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