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  • 1
    Electronic Resource
    Electronic Resource
    Photoaffinity labeling and partial purification of the putative plant receptor for the fungal wilt-inducing toxin, fusicoccin (1989)
    Springer
    Planta 178 (1989), S. 282-290 
    Details
    ISSN: 1432-2048
    Keywords: Fusicoccin ; Fusicoccin-binding protein ; Photoaffinity labeling ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The high-affinity fusicoccin-binding protein (FCBP) was solubilized from plasma-membrane vesicles prepared from leaves of Vicia faba L. by aqueous two-phase partitioning. Conditions for the solubilization of intact FCBP-radioligand complexes were worked out. About 60–70% of the complexes can be solubilized with 50–60 mM nonanoyl-N-methylglucamide in the presence of 1 mg· ml-1 soybean phosphatidylcholine, type IV S, and 20% (v/v) glycerol at pH 5.5. The slow dissociation of the radioligand, 9′-nor-fusicoccin-8′-alcohol-[3H] from the FCBP at low temperatures permits the purification of FCBP-radioligand complexes at 4–10° C by fast protein liquid chromatography on anion-exchange and gel permeation columns. The FCBP, extracted from plasma membranes with cholate and chromatographed in the presence of this detergent, gave an apparent molecular mass (Mr) of 80±20 kDa on gel permeation columns under the conditions used. By comparison of the elution profiles of the fraction most enriched in FCBP-radioligand complexes with polypeptide patterns obtained on sodium dodecyl sulfate-polyacrylamide gels, a polypeptide with an Mr of approx. 34kDa co-separated with the radioactivity profile. A second, faint band of approx. 31 kDa was sometimes also observed co-electrophoresing. Photoaffinity labeling of plasma-membrane vesicles with the new compound 9′-nor-8′[(3,5-[3H]-4-azidobenzoy)ethylenediamine]-fusicoccin ([3H]ABE-FC) and subsequent separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis labeled a single band with an Mr of 35±1 kDa. Labeling in this band was strongly reduced when the membranes were incubated with [3H]ABE-FC in the presence of 0.1–1 μM fusicoccin. From our data, we conclude (i) that the 34-35-kDa polypeptide represents the FCBP and (ii) that in detergent extracts of plasma membranes this polypeptide is probably present as a di- or trimeric structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391855
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  • 2
    Electronic Resource
    Electronic Resource
    Kinetic studies on the redistribution of endogenous growth regulators in gravireacting plant organs (1983)
    Springer
    Planta 158 (1983), S. 339-348 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid (gravitropism) ; Auxin (growth and gravitropism) ; Gibberellin (gravitropism) ; Gravitropism (growth regulators) ; Helianthus ; Vicia ; Zea (gravitropism)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The kinetics of redistribution of endogenous indole-3-acetic acid, cis-abscisic acid and gibberellic acid (+gibberellin A7) in gravistimulated plant organs were followed by immunoassay, during the latent period and the phase of gravitropic curvature. Whereas in maize coleoptile tips, endogenous indole-3-acetic acid accumulated in the lower half of the organ (ratio 65:35, in favour of the lower half) before bending occurred, it was not possible to detect any significant lateral asymmetry of any of the growth regulators assayed in gravitropically reacting root tips of Zea mays L. and Vicia faba L. nor in hypocotyls of Helianthus annus L. Also, no indication was obtained for an exchange of growth regulators between peripheral and central cell layers of the sunflower hypocotyl. Evidence is presented that changes in the properties of the epidermal or subepidermal cell layers located in the lower half of the horizontally placed sunflower hypocotyl are largely responsible for the gravitropic reaction. An alteration in the subcellular compartmentation of IAA may be involved in this process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397336
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  • 3
    Electronic Resource
    Electronic Resource
    Aspirin prevents wound-induced gene expression in tomato leaves by blocking jasmonic acid biosynthesis (1993)
    Springer
    Planta 191 (1993), S. 123-128 
    Details
    ISSN: 1432-2048
    Keywords: Aspirin ; Gene expression ; Jasmonic acid ; Lycopersicon ; Proteinase inhibitor II ; Wounding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Jasmonic acid (JA) and its methyl ester, like mechanical wounding, strongly induce accumulation of proteinase inhibitor II (Pin2) in tomato and potato leaves. In plants, JA is synthesized from α-linolenic acid by a lipoxygenase (LOX)-mediated oxygenation leading to 13-hydroxyperoxylinolenic acid (13-HPLA) which is then subsequently transformed to JA by the action of hydroperoxide-dehydrase activity and additional modification steps. Both the chemical structure as well as the biosynthetic pathway of JA resemble those of the mammalian eicosanoids (prostaglandins and leukotrienes) which are derived from LOX-and cyclooxygenase (COX)-mediated reactions. To assess the role of endogenous JA in the wound response, detached tomato (Lycopersicon esculentum Mill.) leaves were supplied with different LOX and COX inhibitors and the expression of the wound-induced genes for Pin2 (Pin2), cathepsin D inhibitor (Cdi) and threonine deaminase (Td) was analyzed. Lipoxygenase inhibitors as well as some COX inhibitors blocked the wound-induced accumulation of Pin2, Cdi and Td mRNA. Quantitation of endogenous levels of JA showed that aspirin blocks the increase of this phytohormone normally observed as a result of wounding. Linolenic acid and 13-HPLA do not induce the expression of Pin2, Cdi and Td in the presence of aspirin. However, 12-oxo-phytodienoic acid and jasmonic acid are able to overcome the inhibitory effect of this substance. These results strongly indicate that aspirin prevents wound-induced gene activation by inhibiting the hydroxyperoxide-dehydrase activity that mediates the conversion of 13-HPLA to 12-oxo-phytodienoic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240903
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  • 4
    Electronic Resource
    Electronic Resource
    Methyljasmonate and α-linolenic acid are potent inducers of tendril coiling (1991)
    Springer
    Planta 185 (1991), S. 316-322 
    Details
    ISSN: 1432-2048
    Keywords: Bryonia ; Jasmonic acid ; Methyljasmonate ; α-Linolenic acid ; Tendril coiling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A coiling-inducing factor was isolated from tendrils of Bryonia dioica Jacq. and identified by infrared, 1H-, 13C-nuclear magnetic resonance and mass spectrometry as α-linolenic acid. When applied to detached tendrils, exogenous α-linolenic acid, but not linoleic acid or oleic acid, induced tendril coiling. Further investigations showed that metabolites of α-linolenic acid, jasmonic acid and, even more so, methyljasmonate, are highly effective inducers of tendril coiling in B. dioica. Methyljasmonate was most active when administered by air and, in atmospheric concentrations as low as 40–80 nM, induced a full free-coiling response with kinetics similar to mechanical stimulation. Even atmospheric levels as low as 4–5 nM methyljasmonate were still found to be significantly active. Methyljasmonate could be one of the endogenous chemical signals produced in mechanically stimulated parts of a tendril and, being highly volatile, act as a diffusible gaseous mediator spreading through the intracellular spaces to trigger free coiling of tendrils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201050
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  • 5
    Electronic Resource
    Electronic Resource
    Stress-related levels of abscisic acid in guard cell protoplasts of Vicia faba L. (1982)
    Springer
    Planta 154 (1982), S. 24-28 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Guard cell protoplast ; Protoplast (guard cells) ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Levels of abscisis acid (ABA) were determined in isolated guard cell (GCP) and mesophyll cell (MCP) protoplasts of Vicia faba L. in relation to water stress. Incubation of GCP and MCP in 0.4 M or 0.8 M mannitol resulted in an average increase in the level of free abscisic acid (ABA) in the cells of 34% (GCP) and 38% (MCP) within 15–60 min. It is concluded that guard cell protoplasts form ABA in response to osmotic stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385492
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