Publication Date:
2021-05-19
Description:
Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by
establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these
activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic
material between locations and populations when transportation of live fish is restricted. In this study we had a
survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari,
Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six
basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing
temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The
result showd that the best period for sperm collection in this center was from Novamber to January. The best
extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates,
respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C
in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate
(64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and
mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In
conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was
recammended for rainbow trout sperm cryopreservation.
Description:
Iranian Fisheries Science Research Institute
Description:
Published
Keywords:
Sperm Cryopreservation
;
Rainbow trout
;
Extender
;
Cryoprotectant
;
Oncorhynchus mykiss
;
Breeding
;
Genetic
Repository Name:
AquaDocs
Type:
Report
,
Refereed
Format:
54pp.
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