Publication Date:
1994-07-15
Description:
CENP-E is a kinesin-like protein that is transiently bound to kinetochores during early mitosis, becomes redistributed to the spindle midzone at anaphase, and is degraded after cytokinesis. At anaphase, CENP-E may cross-link the interdigitating microtubules in the spindle midzone through a motor-like binding site at the amino terminus and a 99-amino acid carboxyl-terminal domain that bound microtubules in a distinct manner. Phosphorylation of the carboxyl terminus by the mitotic kinase maturation promoting factor (MPF) inhibited microtubule-binding activity before anaphase. Thus, MPF suppresses the microtubule cross-linking activity of CENP-E until anaphase, when its activity is lost.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Liao, H -- Li, G -- Yen, T J -- CA-06927/CA/NCI NIH HHS/ -- GM-44762-02/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1994 Jul 15;265(5170):394-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Fox Chase Cancer Center, Philadelphia, PA 19111.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8023161" target="_blank"〉PubMed〈/a〉
Keywords:
Anaphase
;
Base Sequence
;
Chromosomal Proteins, Non-Histone/*metabolism
;
Chromosomes/*metabolism
;
HeLa Cells
;
Humans
;
Interphase
;
Maturation-Promoting Factor/metabolism
;
Metaphase
;
Microtubules/*metabolism
;
*Mitosis
;
Molecular Sequence Data
;
Phosphorylation
;
Spindle Apparatus/*metabolism
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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