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  • Insertion mutation  (1)
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    Electronic Resource
    Electronic Resource
    The J gene of ϕX174: Isolation and characterization of a J gene mutant (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 72-77 
    Details
    ISSN: 1617-4623
    Keywords: ϕX174 gene J ; Insertion mutation ; Frameshift ; DNA packaging ; DNA synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The J gene protein of bacteriophage ϕX174 is a component of the mature phage. The previous lack of J gene mutants has prevented an in vivo analysis of J protein functions. A ϕX174 mutant was constructed by inserting an 11 nucleotide sequence into the J gene. This mutant, designated insJ, was viable only in the presence of a wild-type J gene carried on a plasmid that could provide J protein. An analysis of DNA synthesis during insJ mutant infection under non-permissive conditions confirmed that the J protein is not required for viral DNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338395
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