ISSN:
1432-0983
Keywords:
Key words Transformation
;
Sulfite
;
Brewing
;
Wine
;
Baking
;
Industrial yeast
;
S. cerevisiae
;
Yeast
;
Sulfur dioxide
;
Selenite
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two S. cerevisiae genes were found to exhibit dominant phenotypes useful for selecting transformants of industrial and laboratory strains of S. cerevisiae. FZF1-4, which confers sulfite resistance, was originally isolated and identified as RSU1-4, but the two genes are shown here to be allelic. Cysteine 57 in wild-type Fzf1p was found to be replaced by tyrosine in Fzf1-4p. Multicopy SSU1, which also confers sulfite resistance, was found to be somewhat less efficient. In both cases, a period of outgrowth in non-selective medium following transformation was found to be necessary. The number of transformants obtained was found to be strain-dependent, and also to depend on the sulfite concentration used during selection. Undesirable background growth of non-transformants was not observed at cell densities as high as 2.5 × 107/plate. In two ura3 laboratory strains where selection for URA3 was applied independently of that for sulfite, the transformation efficiency for sulfite resistance was about 50% that for uracil prototrophy.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050508
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