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  • Industrial Chemistry  (1)
  • alloalleles  (1)
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  • 1
    ISSN: 1573-5028
    Keywords: alloalleles ; cotton ; genome ; Gossypium ; TIP ; tonoplast intrinsic protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tonoplast intrinsic proteins (TIPs) have been implicated in the process of cell elongation, such as occurs in the developing cotton fiber. We have isolated a cDNA clone (997 bp in length) from a cotton (Gossypium hirsutum L.) library which putatively encodes a protein of 248 residues (M r 25079) with 85% identity to Arabidopsis δ-TIP. The derived amino acid sequence included two conserved sequences associated with major intrinsic proteins (SGxHxNPA at residues 78 to 85, NPA residues at 197 to 199) and a cysteine residue at 116 which is reported to bind mercury in Arabidopsis δ-TIP. The polymerase chain reaction was used to generate partial genomic clones of the cotton δ-TIP. In comparison to other genomic TIP sequences, the number (two) and position of the introns were conserved in cotton. Comparing the TIP sequences from cotton revealed two subfamilies, which were consistently distinguished by a Tsp45I restriction site polymorphism. This polymorphism was used to demonstrate that TIP subfamilies were specific to either the A or D genomes of Gossypium. When δ-TIP DNA fragments were amplified from cDNA of fiber 14 days after anthesis, the A and D were found, indicating the presence of δ-TIP transcripts in these elongating cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 34 (1962), S. 669-673 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Die bisher übliche Art der Dimensionierung von Berstscheiben auf Grund von Kurzzeitberstversuchen ist unbefriedigend. Eine Dimensionierung auf Grund von Langzeitberstversuchen bei Betriebstemperatur bringt nur dann eine befriedigende Lösung, wenn Berstscheibenmaterial mit einer flachen Zeitstandcharakteristik verwendet wird. Dann muß aber die Betriebstemperatur an der Berstscheibe sehr niedrig gehalten werden, was jedoch im Chemiebetrieb die Gefahr der Kondensation, Sublimation usw. von Fremdstoffen auf oder vor der Scheibe in sich birgt. Mit Berstscheiben mit definiertem Reißdorn kann vermutlich die Treffsicherheit der Dimensionierung g estegert werden.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
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