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Morphological and cytochemical studies of the effects of ecdysteroids in a lepidopteran cell line (IAL-PID2) (1991)

Cassier, Pierre ; Serrant, Patrick ; Garcia, Régine ; [et al.]

Springer

Cell & tissue research 265 (1991), S. 361-369

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ISSN: 1432-0878

Keywords: Electron microscopy ; Scanning electron microscopy ; Lectin-gold particles ; Cytology ; Glycoproteins ; Imaginal discs ; Tissue culture ; Plodia interpunctella (Insecta)

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The Indian meal-moth cell line, IAL-PID2, established from larval wing discs was examined from the 250th to the 300th passages. The cultured cells retain various structural and functional qualities of epidermal cells. Under hormone-free conditions PID2 cells grow as a monolayer of round or spindle-shaped cells. They appear as weakly active epidermal cells. The endoplasmic reticulum and Golgi apparatus are poorly developed and secretory activity is reduced. Culture conditions resulted in considerable cellular expansions, abundance of storage products (glycogen, lipids), and hypertrophy of the lysosomal system. The PID2 cell line retains the ability to respond to ecdysteroids; 20-hydroxyecdysone treatment (2×10-6 M) triggered morphogenetic and secretory processes. Cells formed pseudoepithelial aggregates interconnected and linked by desmosome-like structures. The hormone-stimulated cells are involved in the biosynthesis of N-acetyl-D-glucosamine-rich glycoproteins. The glycosylation sites were located, by use of WGA-gold particles, on cellular expansions and all along the plasma membrane. The possible significance of these glycoproteins is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00398084

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Ecdysteroid-stimulated synthesis and secretion of an N-acetyl-D-glucosamine-rich glycopeptide in a lepidopterna cell line derived from imaginal discs (1991)

Porcheron, Patrick ; Morinière, Madeleine ; Coudouel, Noëlline ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Archives of Insect Biochemistry and Physiology 16 (1991), S. 257-271

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ISSN: 0739-4462

Keywords: Indian meal moth ; Plodia interpunctella ; chitin ; teflubenzuron ; cycloheximide ; tunicamycin ; 20-hydroxyecdysone ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: Hormone-regulated processing of N-acetyl-D-glucosamine was studied in an insect cell line derived from imaginal wing discs of the Indian meal moth, Plodia interpunctella (Hübner). The cell line, IAL-PID2, responded to treatment with 20-hydroxyecdysone with increased incorporation of GlcNAc into glycoproteins. Cycloheximide and tunicamycin counteracted the action of the hormone. In particular, treatment with 20-hydroxyecdysone resulted in the secretion of a 5,000 dalton N-acetyl-D-glucosamine-rich glycopeptide by the IAL-PID2 cells. Accumulation of this peptide was prevented by the use of teflubenzuron, a potent chitin synthesis inhibitor. A glycopeptide of similar molecular weight was observed in imaginal discs of P. interpunctella treated with 20-hydroxyecdysone in vitro, under conditions that induce chitin synthesis. Although the function of the 5,000 dalton glycopeptide is not known, we believe that the PID2 cell line is a promising model for molecular analysis of ecdysteroid-regulated processing of aminosugars by epidermal cells during insect development.

Additional Material: 8 Ill.