ISSN:
1573-5087
Keywords:
growth regulators
;
In vitro
;
medicinal plant
;
shoot-bud
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract A successful procedure was established for in vitro plant regeneration from callus derived from stem and leaf explants of Centella asiatica on semisolid modified Murashige and Skoog's [7] medium supplemented with 2.0 mg L3 kinetin and 4.0 mg L3 a-naphthaleneacetic acid. The rate of shoot-bud regeneration was the highest (42.8 and 54.3 shoots/culture in stem and leaf derived callus respectively) after 4 weeks of subculture on 4.0 mg L3 6-benzyladenine, 2.0 mg L3 Kn, 0.25 mg L3 a-naphthaleneacetic acid and 20 mg L3 adenine sulfate. Differentiated shoots rooted within 11 days in 1/2 strength MS basal salts supplemented with 0.5 mg L3 indole-3-acetic acid and 2% (w/v) sucrose. About 85% of rooted plantlets were acclimatized and transferred to the greenhouse.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005932313180
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