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  • Immunofluorescence microscopy  (1)
  • Monoclonal antibodies  (1)
  • Spermatozoid  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies to surface and cytoskeletal components of the spermatozoid ofPteridium aquilinum (1988)
    Springer
    Protoplasma 142 (1988), S. 5-14 
    Details
    ISSN: 1615-6102
    Keywords: Basal body ; Cytoskeleton ; Microtubule ; Immunoflu orescence ; Monoclonal antibodies ; Pteridium ; Spermatozoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Detergent extracted spermatozoids of the fernPteridium aquilinum were used as mixed antigen preparations for raising monoclonal antibodies in order to obtain reagents for detecting as yet uncharacterized components of the plant cytoskeleton. Selected antibodies were studied by immunofluorescence microscopy of developing spermatids and mature spermatozoids. Some reacted directly with fixed cells, others required permeabilization treatments with cold methanol or Triton X-100. AntibodiesPas2D9 andPas6D7 bind to glycoprotein antigenic determinants that are exposed on the surface of the plasma membrane. Several antibodies interact with cytoskeletal components.Pas1D3,Pas5D8 andPas5F4 bind to the cytoskeleton of permeabilized cells including the flagella. Three react specifically with the flagellar band or associated components:Pas2G6 reacts with the whole flagellar band but shows a prominent binding to basal bodies,Pas5E2 binds exclusively to basal bodies, andPas5E7 detects mitochondria associated with the flagellar band. Cross-reactions to wheat root tip cells at different stages of the cell cycle are described inMarc andGunning (1988).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273221
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  • 2
    Electronic Resource
    Electronic Resource
    Tubulin isoform usage in maize microtubules (1998)
    Springer
    Protoplasma 204 (1998), S. 235-244 
    Details
    ISSN: 1615-6102
    Keywords: Immunofluorescence microscopy ; Plant microtubules ; Tubulin antibodies ; Tubulin isoforms ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Antibodies specific to two of the maize β-tubulin isoforms and to the three subfamilies of maize α-tubulins were used in immunofluorescence microscopy to determine where and into which microtubule (MT) arrays these tubulin isoforms are incorporated in maize plants. All the tubulins examined appear to be incorporated into MTs in at least some cell types, with the possible exception of subfamily II α-tubulins, which have been found only in the form of diffuse, nonfibrillar staining. Whereas the α-tubulins of subfamily I appear to be used constitutively, others are used much more selectively in the plant, with β2-tubulin found in microtubules only during sexual reproduction. If a particular tubulin is used in the MTs of a given cell type, it appears to be incorporated into all the MT arrays found in the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280327
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    Paper (German National Licenses)
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