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  • 1
    Electronic Resource
    Electronic Resource
    Glutamic acid decarboxylase (GAD)-like immunoreactivity in the pedal ganglion of Mytilus galloprovincialis (1986)
    Springer
    Cell & tissue research 244 (1986), S. 591-593 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; GAD ; Pedal ganglion ; Invertebrate nervous system ; Mytilus galloprovincialis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A substance immunologically related to vertebrate glutamic acid decarboxylase (GAD) has been visualized in the pedal ganglion of Mytilus with the pre-embedding peroxidase-antiperoxidase method, by use of an antiserum raised in sheep against rat brain GAD. The results show that GAD-like immunoreactivity is present both in neuronal perikarya and in nerve fibers. Positive neurons are located mainly among the fibers of the ganglion neuropil at the commissural level, and more rarely close to unreactive cortical cell bodies. Immunoreactive nerve fibers are observed throughout the neuropil and also in cerebropedal and pedal nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212538
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of 5HT-immunoreactivity in the pedal ganglion of Mytilus galloprovincialis (1987)
    Springer
    Cell & tissue research 249 (1987), S. 111-116 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Serotonin ; Mytilus galloprovincialis ; pedal ganglion ; Invertebrate nervous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Serotonergic cell bodies and fibers were identified in the pedal ganglia of Mytilus gattoprovincialis with a serum raised against serotonin and the unlabelled peroxidase-antiperoxidase pre- and post-embedding methods. Examination of Vibratome and serial semithin sections showed that most reactive perikarya are located in the ganglionic cortex, being mainly concentrated at the medial aspect of the postero-dorsal portion of the ganglia. Immunoreactive fibers form a dense network in the neuropil, extend throughout the commissure and run parallel in the nerves and connective tracts. The morphology of serotonin-positive cells compared with that of Golgi-impregnated neurons allows the identification of a main population of unipolar, probably projecting neurons and of smaller multipolar cells likely representing local circuit elements. The ultrastructure of labelled neurons is comparable to that of serotonergic cells described in both vertebrate and invertebrate nervous systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215424
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of collagen types I, III, IV, and fibronectin in the human dermis (1992)
    Springer
    Cell & tissue research 268 (1992), S. 505-511 
    Details
    ISSN: 1432-0878
    Keywords: Dermis ; Collagen fibers ; Extracellular matrix ; Fibronectin ; Immunocytochemistry ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of collagen types I, III, IV, and of fibronectin has been studied in the human dermis by light and electron-microscopic immunocytochemistry, using affinity purified primary antibodies and tetramethylrhodamine isothiocyanate-conjugated secondary antibodies. Type I collagen was present in all collagen fibers of both papillary and reticular dermis, but collagen fibrils, which could be resolved as discrete entities, were labeled with different intensity. Type III collagen codistributed with type I in the collagen fibers, besides being concentrated around blood vessels and skin appendages. Coexistence of type I and type III collagens in the collagen fibrils of the whole dermis was confirmed by ultrastructural double-labelling experiments using colloidal immunogold as a probe. Type IV collagen was detected in all basement membranes. Fibronectin was distributed in patches among collagen fibers and was associated with all basement membranes, while a weaker positive reaction was observed in collagen fibers. Ageing caused the thinning of collagen fibers, chiefly in the recticular dermis. The labeling pattern of both type I and III collagens did not change in skin samples from patients of up to 79 years of age, but immunoreactivity for type III collagen increased in comparison to younger skins. A loss of fibronectin, likely related to the decreased morphogenetic activity of tissues, was observed with age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319157
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  • 4
    Electronic Resource
    Electronic Resource
    Immunocytochemical demonstration of neurotransmitters in the nerve plexuses of the foot and the anterior byssus retractor muscle of the mussel, Mytilus galloprovincialis (1990)
    Springer
    Cell & tissue research 261 (1990), S. 467-476 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin (5-HT) ; Substance P ; GABA ; Immunocytochemistry ; Invertebrate peripheral nervous system ; Mytilus galloprovincialis (Mollusca)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical methods were applied to study the distribution of putative neurotransmitters (5-HT, substance P, GABA, glutamate and aspartate) in the nerve plexuses of the foot and the anterior byssus retractor muscle (ABRM) of Mytilus galloprovincialis (Mollusca, Bivalvia). The foot presents extensive nerve plexuses containing 5-HT and substance P-like immunoreactive material with a similar distribution beneath the surface epithelium, around the vessels and in the glandular regions. Coexistence of the two putative neurotransmitters was observed in a few nerve fibers, Conversely, muscle fibers, both in the foot and in the ABRM, are innervated only by 5-HT-positive fibers, while substance P-like material is present only in the networks of the ABRM epimysial sheath. Immunoreactivity for glutamate and aspartate was not demonstrated, while rare GABA-positive nerve cells and fibers were found only in the foot. The results of this investigation provide a morphological background to previous physiological studies on 5-HT in the nervous system of bivalve molluscs. Moreover, they confirm that the nervous system of Mytilus contains a remarkable amount of a substance related to the vertebrate tachykinin family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00313525
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