ISSN:
1615-6102
Keywords:
Cytoskeleton
;
Immunocytochemistry
;
Microfilaments
;
Microtubules
;
Microtubule organizing center
;
Uromyces phaseoli
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The microtubule and F-actin cytoskeleton of nondifferentiated germlings ofUromyces phaseoli was studied using immunofluorescence methodologies. The microtubules were oriented mostly parallel to the longitudinal axis of the hypha. Microtubule depolymerizing agents, such as cold, demecolcine, griseofulvin and nocodazole, were effective in destroying the microtubule network, but not the F-actin system. Repolymerization of microtubules, following release from these agents, occurred first in the hyphal apices and not near the nuclei or spindle pole bodies. It was concluded that the microtubule nucleating region in such fungal cells is located in the apical regions. Enhanced microtubule arrays were visualized following incubation of the cells in taxol, an agent known to favor microtubule polymerization.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01279730
Permalink
