ISSN:
0173-0835
Keywords:
Biliary proteins
;
High resolution two-dimensional polyacrylamide gel electrophoresis
;
Immobilized pH gradient
;
Gallstone
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Native bile samples, dialyzed against double distilled water, fractionated by Sepharose 2B column chromatography, concentrated by ultrafiltration and lyophilized, were solubilized in lysis buffer and studied by two-dimensional electrophoresis (2-DE) with immobilized pH gradients in the first dimension. We counted 70, and 7 spots in 2-DE patterns of bile from patients with cholesterol gallstones and pigment gallstones, respectively.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150170334
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