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  • 1
    Electronic Resource
    Electronic Resource
    Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter (1996)
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 145-154 
    Details
    ISSN: 1476-5535
    Keywords: lac promoter ; tac promoter ; recombinant DNA ; protein overexpression ; fermentation strategies ; IPTG ; lactose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This review examines factors which influence the expression of foreign proteins inEscherichia coli under the transcriptional control of thelac andtac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-β-d-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569997
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