ISSN:
0268-2605
Keywords:
Hydride generation
;
monomethylmercury
;
dimethylmercury
;
statistics
;
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
This paper describes optimization details of an analytical method development of a hydride-generation procedure for speciation of mercury(II), monomethylmercury cation, dimethylmercury, and diethylmercury using a heated quartz furnace with an atomic absorption spectrophotometer (AAS) as a detector. Typically a new analytical method is developed in steps such as the following: (1) determining analytes individually to confirm retention times and the lack of decomposition during the procedure; (2) comparing peak areas of all analytes as a function of quartz furnace temperature to optimize atomization for AAS detection; (3) conducting factorial experiments to determine which hydride-generation reaction conditions are important in maximizing peak areas and which conditions interact with each other; (4) using the simplex optimization method to give final optimization of reaction conditions. These steps result in conditions that maximize optimized peak areas for analytes while minimizing experimental error.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/aoc.590080723
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