Publication Date:
1985-03-08
Description:
Recent investigations have begun to dissect the number and nature of genetic alterations associated with cancer cells. In the present study, primary human epidermal keratinocytes acquired indefinite life-span in culture but did not undergo malignant conversion in response to infection with a hybrid of adenovirus 12 and simian virus 40. Addition of Kirsten murine sarcoma virus, which contains a K-ras oncogene, to these cells induced morphological alterations associated with the acquisition of neoplastic properties. These findings demonstrate the malignant transformation of human primary epithelial cells in culture and support a multiple-step process for neoplastic conversion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rhim, J S -- Jay, G -- Arnstein, P -- Price, F M -- Sanford, K K -- Aaronson, S A -- New York, N.Y. -- Science. 1985 Mar 8;227(4691):1250-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2579430" target="_blank"〉PubMed〈/a〉
Keywords:
Adenoviruses, Human/*metabolism
;
Animals
;
Cell Transformation, Neoplastic/*metabolism
;
Epithelial Cells
;
Humans
;
Keratins
;
Kirsten murine sarcoma virus/*metabolism
;
Mice
;
Mice, Inbred C3H
;
Mice, Nude
;
Sarcoma Viruses, Murine/*metabolism
;
Sarcoma, Experimental/metabolism
;
Simian virus 40/*metabolism
;
Skin/*cytology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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