Publication Date:
1995-07-28
Description:
Whether neurons express major histocompatibility complex (MHC) class I genes has not been firmly established. The techniques of confocal laser microscopy, patch clamp electrophysiology, and reverse transcriptase-polymerase chain reaction were combined here to directly examine the inducibility of MHC class I genes in individual cultured rat hippocampal neurons. Transcription of MHC class I genes was very rare in neurons with spontaneous action potentials. In electrically silent neurons, transcription was noted, with expression of beta 2-microglobulin under tighter control than in class I heavy chain molecules. Surface expression of class I molecules occurred only in electrically silent neurons treated with interferon gamma. Immunosurveillance by cytotoxic T cells may be focused on functionally impaired neurons.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Neumann, H -- Cavalie, A -- Jenne, D E -- Wekerle, H -- New York, N.Y. -- Science. 1995 Jul 28;269(5223):549-52.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neuroimmunology, Max Planck Institute for Psychiatry, Martinsried, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7624779" target="_blank"〉PubMed〈/a〉
Keywords:
Action Potentials/drug effects
;
Animals
;
Base Sequence
;
Cells, Cultured
;
*Gene Expression Regulation
;
*Genes, MHC Class I
;
Hippocampus/cytology
;
Histocompatibility Antigens Class I/biosynthesis/genetics
;
Interferon-gamma/pharmacology
;
Molecular Sequence Data
;
Patch-Clamp Techniques
;
Polymerase Chain Reaction
;
Pyramidal Cells/cytology/*metabolism/physiology
;
RNA, Messenger/genetics/metabolism
;
Rats
;
Rats, Inbred Lew
;
Tetrodotoxin/pharmacology
;
Transcription, Genetic
;
beta 2-Microglobulin/biosynthesis/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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