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  • Primitive tRNA  (2)
  • High-frequency discharge  (1)
  • Prebiotic synthesis  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Differential coupling efficiency of chemically activated amino acid to tRNA (1984)
    Springer
    Journal of molecular evolution 20 (1984), S. 59-65 
    Details
    ISSN: 1432-1432
    Schlagwort(e): Primitive tRNA ; tRNA ; Aminoacyl imidazole ; Aminoacyl tRNA ; Amino acid charging ; Prebiotic ; Amino acid-polynucleotide interaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Interaction based on possible chemical affinity of an amino acid for tRNA was examined as a model for the aminoacylation of primitive tRNA without aid of an enzyme system. Two types of reaction were carried out and compared. One was the acyl linkage of amino acid to the 5′-terminal phosphate of a tRNA activated as an imidazolide. The other was the incorporation of an amino acid activated as an imidazolide into 2′(3′)-hydroxyl groups of intact tRNA. Both types of reaction indicated that none of the amino acids tested had any selectivity for the tRNAs examined. However, the rates of reaction with a given tRNA were different among amino acids. In the second type of reaction, amino acids were found mainly at loop-out regions of tRNA, but not at either its 5′- or 3′-terminal sites
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02101986
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Synthesis of hydrocarbons under presumed prebiotic conditions using high-frequency discharge (1986)
    Springer
    Journal of molecular evolution 23 (1986), S. 113-118 
    Details
    ISSN: 1432-1432
    Schlagwort(e): Primordial terrestrial atmospheres ; CH4, C2H2, CO2 ; Prebiotic synthesis ; High-frequency discharge ; Hydrocarbons ; Gas chromatography ; Mass Spectrometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Various hydrocarbons were synthesized by high-frequency discharge in a primordial terrestrial model atmosphere. The products were extracted by benzene or methanol and analyzed by GC-MS. The mean carbon chain length of the hydrocarbons formed by the discharge through pure CH4 gas was less than 6. Benzene was also obtained. Some isomers were obtained for each of the hydrocarbons containing a given number of carbons. When a small amount of C2H2 was added to the CH4, longer chain compounds were formed, as compared with discharge in CH4 only. However, when the amount of C2H2 was increased, unextractable high molecular weight compounds were produced. The amounts of products decreased as the mixing ratio of CO2 to CH4 increased. No hydrocarbons were detected when the ratio of CO2/CH4 exceeded 1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02099905
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Enzymatic synthesis and some properties of a model primitive tRNA (1986)
    Springer
    Journal of molecular evolution 23 (1986), S. 320-327 
    Details
    ISSN: 1432-1432
    Schlagwort(e): Primitive tRNA ; Hairpin structure ; Prebiotic ; RNA ligase ; RNase V1 ; Nuclease S1 ; Affinity chromatography ; Anticodon loop ; Anticodon stem ; Amino acid acceptor stem
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A model primitive tRNA with the nucleotide sequence GGCCAAAAAAAGGCCp was synthesized using T4 RNA ligase. The nucleotide sequence of this newly synthesized oligonucleotide was confirmed by ladder analysis of several enzymatic digestion products. The secondary structure of the oligonucleotide was examined by comparison of the products of its digestion by single- and double-strand-specific nucleases with those of the digestion of the intermediate oligonucleotide GGCCAAAAAAAOH. The results indicated that the two GGCC segments of the 5′ and 3′ ends of the model tRNA may form base pairs in solution. The same conclusion was derived from the result of affinitycolumn chromatography of the model oligonucleotide. When32pGGCCAAAAAAAGGCCOH was passed through a poly(U)-agarose column, about 70% of the applied sample bound to the poly(U)-agarose. In contrast, when the model oligonucleotide was passed through a poly(C)-agarose column, only 15% of the sample bound to the poly(C)-agarose. These results indicate that the newly synthesized oligonucleotide adopts a hairpin structure in solution. Two aspects of a potential biological activity of the synthetic model tRNA were examined. It was found that the oligonucleotide can bind to poly(U)-programmed 30S ribosomes and is recognized by Qβ replicase as a template for RNA synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02100641
    Standort Signatur Erwartet Verfügbarkeit
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