ISSN:
1573-5044
Keywords:
cell suspensions
;
embryogenesis
;
Hevea brasiliensis
;
plant regeneration
;
protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Embryogenic cell suspensions of rubber derived from immature inflorescences and inner integuments of immature fruits released 3.1 ± 0.2 × 107 protoplasts g-1 f. wt. (mean ± s.e.m, n = 10) and 3.2 ± 0.2 × 107 protoplasts g-1 f. wt., with mean viabilities of 83 ± 2% and 77 ± 8%, respectively. Sustained mitotic division was observed only when protoplasts were cultured in KPR liquid medium on nitrocellulose membranes overlying the same semi-solid medium containing Lolium multiflorum nurse cells. Protoplast-derived cell colonies were produced within 2 months of culture. Protoplast-derived cell colonies proliferated, upon subculture to MS-based regeneration medium, with 40% of the protoplast-derived calli developing somatic embryos. The latter germinated into plants on the same medium after 3 months of culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006494404224
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