ISSN:
1432-0983
Keywords:
Key words Trichostatin A
;
Repression
;
Schizosaccharomyces pombe
;
Heterochromatin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric, i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h 90 cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050436
Permalink
