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  • Articles  (3)
  • Helianthus annuus  (2)
  • Ribosomal-operon  (1)
  • 1
    ISSN: 1432-0983
    Keywords: Chloroplast ; Euglena ; Ribosomal-operon ; Unequal-recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Modifications of the chloroplast genome have been investigated in 35 mutants ofEuglena gracilis which have antibiotic-resistant chloroplasts. Whereas each molecule of wild-type chloroplast DNA has three tandemly repeated ribosomal operons (rrn), endonuclease restriction and electron microscopy studies demonstrate that the number of chloroplastrrn is frequently modified in the mutants: only one operon is remaining in 16 of the 35 mutants; among the 14 streptomycinresistant strains, nine have one operon, one has 2 operons, and another one has 4 operons. Modifications of the number of chloroplastrrn may be explained by unequal recombinations between theserrn, arising either between the operons of the same molecule or between the operons of two molecules. These recombinations might occur during a repair process following the mutagenic treatment. Some loci for antibiotic resistance might be located at the recombination point on therrn.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-9788
    Keywords: Helianthus annuus ; QTL ; resistance ; RFLP ; Sclerotinia scerotorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Restriction fragment length polymorphism and isoenzyme markers were used to investigate quantitative trait loci involved in sunflower resistance to mycelial extension of Sclerotinia sclerotiorum on leaves and capitula. Seed weight, oil content and flowering data were also evaluated. Four quantitative trait loci were demonstrated for leaf resistance and two for capitulum resistance. One of these zones appears involved in resistance to both types of S. sclerotiorum attack while the others appear specific for resistance of one part of the plant. Two quantitative trait loci were detected for seed weight, three for oil content and three for flowering date. Individual quantitative trait loci explained 9% to 48% of the phenotypic variability, confirming the polygenic basis of the quantitative traits studied. Overall, the quantitative trait loci explain 60% of the genetic variation for leaf resistance and 38% for capitulum resistance to S. sclerotiorum. One linkage group is particularly interesting since it includes quantitative trait loci for all the five quantitative traits measured. Hypotheses for linkage versus pleiotropy and consequences of all the results in resistance breeding are discussed.
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  • 3
    ISSN: 1573-5060
    Keywords: bulked segregant analysis ; Helianthus annuus ; linkage ; Plasmopara halstedii ; resistance genes ; RFLP ; sunflower ; downy mildew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Pl6 locus in the inbred sunflower (Helianthus annuus L.) line HA335 giving resistance to French races of downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni. was localized by molecular techniques. A bulked segregant analysis was made on the F2 progeny from a cross between this line and H52, a downy mildew susceptible line. The resistance gene in HA335 was found to have the same linked RFLP marker loci as those determined for Pl1 (resistance to race 1 in the line RHA266) on linkage group 1 of the consensus RFLP map of the cultivated sunflower. Pl1 and Pl6 thus appear either to be allelic or closely linked. The implications for sunflower breeding are discussed.
    Type of Medium: Electronic Resource
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