ISSN:
1573-9686
Keywords:
Flow chamber
;
Hele-Shaw flow
;
Cell adhesion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
Abstract A new paralle plate flow chamber that has a linear variation of shear stress, starting from a predetermined maximum value at the entrance and falling to zero at the exit, has been designed and tested. This is in contrast to the usual rect-angular channel plan which produces a constant shear stress over the entire length. The new design is based on the theory of Hele-Shaw flow between parallel plates. To verify the efficacy of the flow channel, the effect of fluid shear stress on platelet adhesion to a fibrinogen-coated glass surface was tested. The percentage of attached platelets after 5 min of shear stress is shown to be a function of shear stress. With this new flow chamber, cell-cell interactions can be studied efficiently over a wide range of shear stress using a single run at constant discharge.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02368167
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