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  • H ion efflux  (3)
  • exchangeable Ca  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 84 (1985), S. 403-415 
    ISSN: 1573-5036
    Keywords: Agar-pH indicator technique ; Cation-anion balance ; H ion efflux ; HCO3 ; ion efflux ; Nitrate nutrition ; Nitrate reductase ; Root growth ; Split root system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Changes in pH around the roots of young rape plants (Brassica napus cv. emerald) were studied using a nutrient film technique that allowed part or whole of the root system to be subjected to specific nutrient treatments. The rapidity and direction of pH change was assessed by imbedding absorbing roots in a thin film of agar containing bromocresol purple. When nitrate-fed plants were deprived of all sources of nitrogen at 15 or 17 days old, the release of H ions from the roots was immediate and uniformly distributed over the root length. When nitrate was witheld from half of the root system of nitrate-fed plants, the roots deprived of nitrate immediately started to produce H ions even though the nitrate-fed half of the root system continued to supply the whole of the plant with nitrate. However, the rate of H ion production in plants partly supplied with NO3 was less than in plants completely deprived of NO3. It is suggested that malate produced in the shoots, following nitrate reduction, may be redistributed to the roots deprived of nitrate. There, HCO3 produced by the decarboxylation of the malate masks some of the expected H ion efflux.
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  • 2
    ISSN: 1573-0867
    Keywords: Anion exchange resin ; Bray 1 ; cation exchange resin ; Colwell ; exchangeable Ca ; P dissolution ; P fractionation ; P sorption capacity ; P tests ; Phosphate rocks ; Olsen ; Truog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The objective of this work was to develop and evaluate a soil test suitable for estimating the phosphorus status of soils whether they were fertilized with soluble or sparingly soluble P fertilizers or both. Four New Zealand soils of contrasting P sorption capacity and exchangeable Ca content were incubated alone or with monocalcium phosphate (MCP), reactive North Carolina (NC) phosphate rock or unreactive Florida (FRD) rock, at 240 mg P kg−1 soil, to allow the P sources of different solubilities to react with each soil and provide soil samples containing different amounts of extractable P, Ca and residual phosphate rock. The phosphorus in the incubated soils was fractionated into alkali soluble and acid soluble P fractions using a sequential extraction procedure to assess the extent of phosphate rock dissolution. Eight soil P tests [three moderately alkaline — Olsen (0.5M NaHCO3) modified Olsen (pretreatment with 1M NaCl) and Colwell; three acid tests — Bray 1, modified Bray 1 and Truog; and two resin tests — bicarbonate anion exchange resin (AER) and combined AER plus sodium cation exchange resin (CER)] were assessed in their ability to extract P from the incubated soils. The 0.5M NaHCO3 based alkaline tests could not differentiate between the Control and FRD treatments in any soil nor between the Control, NC and FRD treatments in the high P sorption soils. The acid extractants appeared to be affected by the P sorption capacity of the soil probably because of reabsorption of dissolved P in the acid medium. The AER test gave results similar to Olsen. Only the combined AER + CER test extracted P in amounts related to the solubility of the P sources incubated with each soil. Furthermore, when soil samples were “spiked” with FRD and NC and extracted immediately, the P extracted by the AER + CER test, over and above the control soils, increased with the amount and chemical reactivity of the rocks. There was no extraction of rock P by any of the alkaline extractions. Increases in the amounts of P extracted (ΔP) by each soil test from the fertilized soils, over and above the control soils were compared with the amounts ofP dissolved from the fertilizers during incubation (measured by P fractionation). Soil P sorption capacity had least influence on the amounts of P extracted by the AER + CER and Colwell tests. However, the Colwell test was unable to differentiate between all P sources in all four soils and suffered from the disadvantage of producing coloured extracts. The AER + CER test appeared to have the potential to assess the available P status of soils better than the other tests used because of its ability to extract a representative portion of residual PR (in accordance with the amount and reactivity) and dissolved P, and thus to differentiate between fertilizer treatments in all four soils.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 24 (1990), S. 173-180 
    ISSN: 1573-0867
    Keywords: Phosphate ; resin beads ; resin membranes ; resin-strips ; anion exchange resin ; cation exchange resin ; P sorption capacity ; exchangeable Ca ; phosphate rocks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A simplified procedure for determining the amount of phosphate (P) extracted from soils by ion exchange resin membranes is reported. Strips of anion (HCO 3 - form) and cation (Na+ form) exchange membrane were shaken with suspensions of soil in deionised water for 16–17 hours. After shaking, the strips were thoroughly rinsed in deionised water before the phosphate retained on the anion exchange resin strip was determined by shaking the strip directly with phosphate reagent. Compared to the common use of resin beads in nylon mesh bags, this resin membrane procedure is simpler, more convenient, and because an elution step is omitted, less time consuming. The mixed resin membrane method for soil phosphate extraction was compared to the use of resin bags on four New Zealand soils, contrasting in P sorbing capacity and exchangeable calcium. The soils were preincubated with and without 240 mg P kg−1 soil with three P sources of different solubilities. The resin strips extracted amounts of P which were closely correlated (R2 = 0.972) with that extracted by the resin bags. The amounts of P extracted by the mixed resin procedure were in proportion to the solubility of the P sources in each soil.
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  • 4
    ISSN: 1573-0867
    Keywords: Dry matter yield ; exchangeable Ca ; phosphate rocks ; P sorption capacity ; relative agronomic effectiveness ; substitution ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Six phosphate rocks (PRs) of varying reactivities were compared with monocalcium phosphate (MCP) in a glasshouse experiment growing perennial ryegrass (Lolium perenne cv. Nui) as the test plant on four soils of contrasting P sorption capacity and exchangeable Ca. The cumulative dry matter yield over 10 harvests showed a significant response to P application in all soils. Based on relative yield and P uptake, MCP was the most effective P fertilizer followed by the reactive phosphate rocks, which were superior to the unreactive rocks in all soils. The relative agronomic effectiveness (RAE) and substitution ratio (SR) of individual PR fertilizers, calculated with respect to MCP using the methods of ‘vertical’ and ‘horizontal’ comparison, respectively, were similar over a range of fertilizer rate. There was a decline or slight increase in the performance of PRs with time in the low P sorption soils but a consistent increase in the high P sorption soils. Some initial influence of exchangeable Ca content of the soils on the relative performance of PRs was also observed. Generally the PRs performed better in high P sorption soils than low P sorption soils and in low exchangeable Ca soils than high exchangeable Ca soils.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 105 (1988), S. 247-256 
    ISSN: 1573-5036
    Keywords: Brassica napus ; cation-anion balance ; H ion efflux ; phosphate nutrition ; root growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Changes in pH around the roots of young rape plants were studied using a nutrient film technique which allowed either part or all of the root system to be subjected to specific nutrient treatments. The rapidity and direction of change of pH was assessed by embedding absorbing roots in a thin layer of agar containing bromocresol purple. Measurements were also made with a pH microelectrode placed next to the roots. Phosphate-fed plants were deprived of phosphate when 14 days old. Patterns of pH changes round the deprived roots were the same as with phosphate-fed plants until the plants had been deprived of P for three days, when H ion efflux started in the terminal portions of the roots. The lengths of root producing acid and amounts of H ion both increased as the plants became more P deficient. Both P fed and P deprived roots produced HCO3 ions but the net amount of HCO3 ion produced by the P deficient roots fell as did nitrate uptake rates. Cation-anion balances measured at the end of the experiment showed that uptake of all anions and K decreased in the P deprived plants but uptake of Ca and Mg were little altered. This resulted in a smaller ratio of anions to cations absorbed which was reflected in the reduced HCO3 ion efflux.
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  • 6
    ISSN: 1573-5036
    Keywords: Brassica napus ; H ion efflux ; nitrate accumulation ; nitrate reductase ; phosphate nutrition ; xylem exudation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Levels of nitrate reductase activity (N.R.A.) were measured in shoots and roots of P sufficient and P deficient rape plants and changes in N.R.A. examined in relation to the onset of H ion efflux from the roots. Rates of xylem exudation were measured and the sap analysed for nitrate, amino-N and phosphate content. The optimum concentration of phosphate in the leaves for N.R.A. was about 0.7%. Both high and low concentrations of phosphate within the leaves inhibited N.R.A in those leaves. This inhibition of N.R.A led to the accumulation of nitrate in the older parts of the shoots of P sufficient plants. Less accumulation of nitrate occurred in the P deficient plants since nitrate uptake by the plants decreased before any fall in N.R.A. Xylem exudation rates halved within 18 hours of depriving the plants of phosphate, and, since the composition of the sap remained constant, this indicated a reduced flux of nitrate into the xylem. The rate of xylem exudation continued to fall and by the end of the experiment was approximately one tenth of the rate in the P sufficient plants. The onset of H ion efflux from the terminal portions of the root preceded any effect on N.R.A by 2 days.
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