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  • Polymer and Materials Science  (2)
  • Guanosine 2'-monophosphate  (1)
  • OmpA signal peptide  (1)
  • 1
    ISSN: 0378-1119
    Keywords: HU ; OmpA signal peptide ; Recombinant DNA ; cassette mutagenesis ; prokaryotic expression
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 1073 (1991), S. 357-365 
    ISSN: 0304-4165
    Keywords: Association rate ; Chemical relaxation ; Guanosine 2'-monophosphate ; Guanosine 3'-monophosphate ; Temperature-jump
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 20 (1989), S. 416-421 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Effective Sealing Area of Osculating Metallic GasketsThe question of importance in the design of flanged joints using metallic osculating gaskets is the contact area and the effective width of the gasket. For the three important sealing materials armco iron, austenitic steel X 10 CrNiTi 189 and high-temperature steel 12 CrMo 195 the dependence of the effective gasket width on pressure was determined experimentally through variation of the radius of curvature. The comparison of the measured data with existing numerical approaches is not congruent to a sufficient degree. New formulations of the relations, which correspond better to the experimentally determined results, are developed on the basis of material mechanics.
    Notes: Bei der Auslegung von Flanschverbindungen unter Verwendung von metallischen Schmiegungsdichtungen stellt sich die Frage nach der Auflagefläche bzw. der wirksamen Breite der Dichtungen. Unter Variation des Schmiegungsradius wird für drei wichtige Dichtungswerkstoffe (Armcoeisen, austenitischer Stahl X 10 CrNi-Ti 189 und warmfester Stahl 12 CrMo 195) die Abhängigkeit der wirksamen Dichtungsbreite von der Pressungskraft auf experimentellem Wege ermittelt. Der Vergleich der Meßwerte mit bestehenden Berechnungsansätzen ergibt i.a. keine hinreichende Übereinstimmung. Es werden neue, werkstoffmechanisch fundierte Formulierungen der Zusammenhänge entwickelt, die den experimentell ermittelten Gegebenheiten besser entsprechen.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Biopolymers 24 (1985), S. 1147-1156 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The anisotropic rotational motion of the backbone and the side chains of poly(L-glutamic acid) in the α-helical structure was investigated using the 13C-T1 and T2 relaxation times of all carbon atoms with directly attached protons, obtained at a 13C-Larmor frequency of 67.89 MHz. The evaluation of the nmr data was carried out according to the previously derived anisotropic diffusion model, in which the macromolecule is considered a rigid rod. The rotation of the backbone is characterized by two diffusion constants, D1 and D3, describing the rotation perpendicular to and around the symmetry axis. The additional internal motion of the Cβ-methylene group is described as a jump process with a jump rate, k1, between two allowed rotametric states. Steric considerations indicate that the occupation of the third rotameric position is forbidden. The rotation of the Cγ-methylene group is decribed as a one-dimensional diffusion process around the Cβ-Cγ bond. Investigation of the temperature dependence of the relaxation parameters led to the temperature dependence of the dynamic parameters. Activation energies were determined from these data. The dynamic parameters obtained for poly(L-glutamic acid) at 291 K are compared with the corresponding results of a previous study of poly(L-lysine).The development of an anisotropic diffusion model for the motions of the rod-shaped poly(L-lysine) α-helix and its application to the interpretation of the 13C-relaxation data of this molecule have already been published previously. In this model, both the overall molecular tumbling and the various internal motions have been characterized by diffusion constants or jump rates typical for each process. These dynamic parameters can be calculated from the spin-lattice relaxation times, the spin-spin relaxation times and the NOE factors of the Cα, Cβ, and Cγ nuclei of the polypetide.In the present paper, we describe the application of the above-mentioned dynamic model to the interpretation of 13C-relaxation studies of a further homopolypeptide, poly(L-glutamic acid), in the α-helical structure. Furthermore, we studied the temperature dependence of the relaxation times of this polymer and determined the anisotropic diffusion parameters at each temperature. From their temperature dependence and from comparison of our present results with the data of our previous study of poly(L-lysine), we were able to derive new insights into the intramolecular diffusion processes and the excitation of various motions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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