ISSN:
1573-5044
Keywords:
Gossypium
;
interspecific hybrid
;
ovule culture
;
triploid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An interspecific hybrid of the sexually incompatible species G. hirsutum cv. Laxmi and G. arboreum cv. Jyoti was obtained through in ovulo embryo culture. Eightto twelve-day-old ovules were excised and cultured on Beasley and Ting's medium supplemented with Indol-3 acetic acid (5×10-6 to 7×10-6 M), Kinetin (5×10-6 to 5×10-8 M), Gibberellic acid (5×10-7 to 5×10-9M), Ammonium chloride (5 to 15mM) and Casein hydrolysate (50 to 200mg/l) added individually and in various combinations along with sucrose. No single medium was adequate to ensure complete development of the fertilized ovules to plantlets, thus necessitating a sequential five step transfer to different media. Cytological studies confirmed the hybrid nature of the plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040006
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