ISSN:
1612-1112
Keywords:
Liquid chromatography
;
Bile acids
;
Glyco-conjugates
;
Tauro-conjugates
;
Electrochemical detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A rapid and sensitive method has been developed for the simultaneous determination of free bile acids and its glyco-and tauro-conjugates by high-performance liquid chromatography (HPLC). These components were separated on a resin-based reversed-phase column, with a gradient of acetonitrile in dilute sodium hydroxide solution. The detection was performed at a gold electrode with a sequence of three pulses, which enabled the selective detection of hydroxyl-containing compounds, without fouling of the electrode. Detection limits ranged from 30 to 60 pmol oncolumn. Linear calibration graphs were obtained for bile acid solutions of 0.6 to 14 μmol/l. The method was applied to the determination of bile acids in culture media, bile, and ileal fluid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02279474
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